- About Us
- Career Center
- Nano-Social Network
- Nano Consulting
- My Account
Dental researchers use Malvern Zetasizer Nano to characterize tooth enamel made in the laboratory
Dr Vuk Uskokovic, from the Department of Preventive and Restorative Dental Sciences, School of Dentistry at the University of California, San Francisco (UCSF), is involved in a study that aims to mimic the growth of tooth enamel in the laboratory. A key analytical tool in this NIH-funded project is the Zetasizer Nano particle characterization system from Malvern Instruments.
Harnessing the combined dynamic light scattering (DLS) and zeta-potential measurement capabilities of the instrument, Dr Uskokovic and his colleagues have been able to characterize the interaction between amelogenin protein, which makes up 90% of the enamel matrix, and the mineral component, hydroxyapatite. Self assembly of this particular protein is thought to be responsible for guiding the formation of enamel crystals.
While dental techniques are often highly sophisticated, those available for restoring damaged dental tissue are less than perfect. Consequently there is a requirement for approaches that minimise tissue loss. According to Dr Uskokovic, if we understand how enamel forms naturally we can use the same compounds to rebuild the damaged enamel.
In a paper entitled, ‘Zeta-potential and Particle Size Analysis of Human Amelogenins', (Uskokovic et al., J Dent Res 89(2):149-153, 2010) Dr Uskokovic and his colleagues deliver results that suggest that: "zeta-potential may be used as a control parameter in replicating the assembly of amelogenins in vitro." The authors also note that: "…the meaning of the correlations established [in the paper] between zeta-potential and particle-particle attraction could be potentially applied to self-assembling proteins in general."
When asked why he selected Malvern's Zetasizer Nano for his work, Dr Uskokovic said: "I had previous experience with the Zetasizer Nano, albeit with more robust inorganic substances, so when asked to purchase a protein particle characterization system I knew it might be a good candidate. In a side by side trial with a competitive product, there was no contest. Results from the Zetasizer Nano were both more reliable and more robust."
Dr Uskokovic continued: "Proteins are sensitive to electric fields and during zeta potential measurements proteins are known to accidentally ‘cook' if you're not careful. To achieve usable results, it was therefore necessary to fine tune our procedures to overcome these challenges and it was a huge advantage to have the experts at Malvern Instruments so readily available to answer questions and deliver detailed guidance. As a result, I am now able to pass on this tutelage to students volunteering in our lab."
Having completed the initial study covering the characterization of amelogenin protein, including its proteolytic cleavage products, as published in the Journal of Dental Research, Dr Uskokovic is currently awaiting publication of a more comprehensive paper focusing on the interaction between amelogenin and its mineral counterpart in enamel.
Malvern Instruments Zetasizer Nano delivers high quality DLS and zeta potential measurements in a single instrument. The combination of these techniques, and the reliability of results, makes the instrument ideal for protein characterization. For further information, please visit: www.malverninstruments.com/zetasizer
Malvern, Malvern Instruments and Zetasizer are registered trademarks of Malvern Instruments Ltd
About Malvern Instruments
Malvern Instruments is a market leader in measuring performance controlling material properties. These include particle size, particle shape, zeta potential, molecular weight, size and conformation, rheological properties and chemical distribution. Malvern delivers the systems, support and expertise that ensure the analytical integrity and productivity needed to drive research, development and manufacturing.
Malvern’s measurement solutions for scientists, technologists and engineers advance continually through customer collaboration. Complementary materials characterization systems deliver inter-related measurements that reflect the complexities of particulates and disperse systems, nanomaterials and macromolecules. Combining intelligently implemented technologies with in-depth industry applications knowledge and support, Malvern provides customers with the competitive advantage they demand.
Headquartered in Malvern, UK, Malvern Instruments has subsidiary organizations in all major European markets, North America, China, Korea and Japan, a joint venture in India, a global distributor network and applications laboratories around the world. www.malvern.com
For more information, please click here
For press information, please contact:
Great North Road
Tel: +44 (0)1480 479280
Fax: +44 (0)1480 470343
Malvern Instruments Inc.
117 Flanders Road
Tel: +1 508 768 6400
Fax: +1 508 768 6403
Please send sales enquiries to:
Malvern Instruments Ltd
Enigma Business Park
Tel: +44 (0) 1684 892456
Fax: +44 (0) 1684 892789
Copyright © Malvern InstrumentsIf you have a comment, please Contact us.
Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.
|Related News Press|
News and information
New conductive ink for electronic apparel June 25th, 2015
Giving atoms their marching orders: Highly homogeneous nanotube enforces single-file flow of atoms in gas diffusion. Direct comparison of single-file and Fickian diffusion possible with new system described by researchers at the University of South Carolina and University of Flor June 24th, 2015
Graphene reduces wear of alumina ceramic March 26th, 2015