Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Building teeth

Abstract:
Dental researchers use Malvern Zetasizer Nano to characterize tooth enamel made in the laboratory

Building teeth

Malvern, UK | Posted on October 20th, 2010

Dr Vuk Uskokovic, from the Department of Preventive and Restorative Dental Sciences, School of Dentistry at the University of California, San Francisco (UCSF), is involved in a study that aims to mimic the growth of tooth enamel in the laboratory. A key analytical tool in this NIH-funded project is the Zetasizer Nano particle characterization system from Malvern Instruments.

Harnessing the combined dynamic light scattering (DLS) and zeta-potential measurement capabilities of the instrument, Dr Uskokovic and his colleagues have been able to characterize the interaction between amelogenin protein, which makes up 90% of the enamel matrix, and the mineral component, hydroxyapatite. Self assembly of this particular protein is thought to be responsible for guiding the formation of enamel crystals.

While dental techniques are often highly sophisticated, those available for restoring damaged dental tissue are less than perfect. Consequently there is a requirement for approaches that minimise tissue loss. According to Dr Uskokovic, if we understand how enamel forms naturally we can use the same compounds to rebuild the damaged enamel.

In a paper entitled, ‘Zeta-potential and Particle Size Analysis of Human Amelogenins', (Uskokovic et al., J Dent Res 89(2):149-153, 2010) Dr Uskokovic and his colleagues deliver results that suggest that: "zeta-potential may be used as a control parameter in replicating the assembly of amelogenins in vitro." The authors also note that: "…the meaning of the correlations established [in the paper] between zeta-potential and particle-particle attraction could be potentially applied to self-assembling proteins in general."

When asked why he selected Malvern's Zetasizer Nano for his work, Dr Uskokovic said: "I had previous experience with the Zetasizer Nano, albeit with more robust inorganic substances, so when asked to purchase a protein particle characterization system I knew it might be a good candidate. In a side by side trial with a competitive product, there was no contest. Results from the Zetasizer Nano were both more reliable and more robust."

Dr Uskokovic continued: "Proteins are sensitive to electric fields and during zeta potential measurements proteins are known to accidentally ‘cook' if you're not careful. To achieve usable results, it was therefore necessary to fine tune our procedures to overcome these challenges and it was a huge advantage to have the experts at Malvern Instruments so readily available to answer questions and deliver detailed guidance. As a result, I am now able to pass on this tutelage to students volunteering in our lab."

Having completed the initial study covering the characterization of amelogenin protein, including its proteolytic cleavage products, as published in the Journal of Dental Research, Dr Uskokovic is currently awaiting publication of a more comprehensive paper focusing on the interaction between amelogenin and its mineral counterpart in enamel.

Malvern Instruments Zetasizer Nano delivers high quality DLS and zeta potential measurements in a single instrument. The combination of these techniques, and the reliability of results, makes the instrument ideal for protein characterization. For further information, please visit: www.malverninstruments.com/zetasizer

Malvern, Malvern Instruments and Zetasizer are registered trademarks of Malvern Instruments Ltd

####

About Malvern Instruments
Malvern Instruments is a market leader in measuring performance controlling material properties. These include particle size, particle shape, zeta potential, molecular weight, size and conformation, rheological properties and chemical distribution. Malvern delivers the systems, support and expertise that ensure the analytical integrity and productivity needed to drive research, development and manufacturing.

Malvern’s measurement solutions for scientists, technologists and engineers advance continually through customer collaboration. Complementary materials characterization systems deliver inter-related measurements that reflect the complexities of particulates and disperse systems, nanomaterials and macromolecules. Combining intelligently implemented technologies with in-depth industry applications knowledge and support, Malvern provides customers with the competitive advantage they demand.

Headquartered in Malvern, UK, Malvern Instruments has subsidiary organizations in all major European markets, North America, China, Korea and Japan, a joint venture in India, a global distributor network and applications laboratories around the world. www.malvern.com

For more information, please click here

Contacts:
For press information, please contact:

Trish Appleton
Kapler Communications
Knowledge Centre
Wyboston Lakes
Great North Road
Wyboston
Bedfordshire
MK44 3BY
UK
Tel: +44 (0)1480 479280
Fax: +44 (0)1480 470343

USA contact:

Marisa Fraser
Malvern Instruments Inc.
117 Flanders Road
Westborough
MA 01581-1042
USA
Tel: +1 508 768 6400
Fax: +1 508 768 6403


Please send sales enquiries to:

Alison Vines
Malvern Instruments Ltd
Enigma Business Park
Grovewood Road
Malvern
Worcestershire
WR14 1XZ
UK
Tel: +44 (0) 1684 892456
Fax: +44 (0) 1684 892789

Copyright © Malvern Instruments

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Spin current detection in quantum materials unlocks potential for alternative electronics October 15th, 2017

Quantum manipulation power for quantum information processing gets a boost: Improving the efficiency of quantum heat engines involves reducing the number of photons in a cavity, ultimately impacting quantum manipulation power October 14th, 2017

Injecting electrons jolts 2-D structure into new atomic pattern: Berkeley Lab study is first to show potential of energy-efficient next-gen electronic memory October 13th, 2017

The secret to improving liquid crystal's mechanical performance: Better lubricating properties of lamellar liquid crystals could stem from changing the mobility of their structural dislocations by adding nanoparticles October 13th, 2017

Govt.-Legislation/Regulation/Funding/Policy

Rice U. study: Vibrating nanoparticles interact: Placing nanodisks in groups can change their vibrational frequencies October 16th, 2017

Spin current detection in quantum materials unlocks potential for alternative electronics October 15th, 2017

Injecting electrons jolts 2-D structure into new atomic pattern: Berkeley Lab study is first to show potential of energy-efficient next-gen electronic memory October 13th, 2017

Rice U. lab surprised by ultraflat magnets: Researchers create atom-thick alloys with unanticipated magnetic properties October 13th, 2017

Self Assembly

Physicists gain new insights into nanosystems with spherical confinement: Enormous potential for the targeted delivery of pharmaceutical agents and the creation of tailored nanoparticles July 27th, 2017

Oddball enzyme provides easy path to synthetic biomaterials May 17th, 2017

Nanotubes that build themselves April 14th, 2017

Nanocages for gold particles: what is happening inside? March 16th, 2017

Announcements

Rice U. study: Vibrating nanoparticles interact: Placing nanodisks in groups can change their vibrational frequencies October 16th, 2017

Spin current detection in quantum materials unlocks potential for alternative electronics October 15th, 2017

Quantum manipulation power for quantum information processing gets a boost: Improving the efficiency of quantum heat engines involves reducing the number of photons in a cavity, ultimately impacting quantum manipulation power October 14th, 2017

The secret to improving liquid crystal's mechanical performance: Better lubricating properties of lamellar liquid crystals could stem from changing the mobility of their structural dislocations by adding nanoparticles October 13th, 2017

Tools

Nanometrics Announces Preliminary Results for the Third Quarter of 2017: Quarterly Results Impacted by Delays in Revenue Recognition on Multiple Systems into Japan October 12th, 2017

Seeing the next dimension of computer chips: Researchers image perfectly smooth side-surfaces of 3-D silicon crystals with a scanning tunneling microscope, paving the way for smaller and faster computing devices October 11th, 2017

Quorum announces new customer support and demonstration facilities for users worldwide October 10th, 2017

Graphene forged into three-dimensional shapes September 26th, 2017

Dental

A Tougher Tooth: A new dental restoration composite developed by UCSB scientists proves more durable than the conventional material August 22nd, 2017

New technology can detect tiny ovarian tumors: 'Synthetic biomarkers' could be used to diagnose ovarian cancer months earlier than now possible April 10th, 2017

New stem cell technique shows promise for bone repair January 25th, 2017

Nanocellulose in medicine and green manufacturing: American University professor develops method to improve performance of cellulose nanocrystals November 7th, 2016

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project