Nanotechnology Now

Heifer International

Wikipedia Affiliate Button


Home > Press > Building teeth

Dental researchers use Malvern Zetasizer Nano to characterize tooth enamel made in the laboratory

Building teeth

Malvern, UK | Posted on October 20th, 2010

Dr Vuk Uskokovic, from the Department of Preventive and Restorative Dental Sciences, School of Dentistry at the University of California, San Francisco (UCSF), is involved in a study that aims to mimic the growth of tooth enamel in the laboratory. A key analytical tool in this NIH-funded project is the Zetasizer Nano particle characterization system from Malvern Instruments.

Harnessing the combined dynamic light scattering (DLS) and zeta-potential measurement capabilities of the instrument, Dr Uskokovic and his colleagues have been able to characterize the interaction between amelogenin protein, which makes up 90% of the enamel matrix, and the mineral component, hydroxyapatite. Self assembly of this particular protein is thought to be responsible for guiding the formation of enamel crystals.

While dental techniques are often highly sophisticated, those available for restoring damaged dental tissue are less than perfect. Consequently there is a requirement for approaches that minimise tissue loss. According to Dr Uskokovic, if we understand how enamel forms naturally we can use the same compounds to rebuild the damaged enamel.

In a paper entitled, ‘Zeta-potential and Particle Size Analysis of Human Amelogenins', (Uskokovic et al., J Dent Res 89(2):149-153, 2010) Dr Uskokovic and his colleagues deliver results that suggest that: "zeta-potential may be used as a control parameter in replicating the assembly of amelogenins in vitro." The authors also note that: "…the meaning of the correlations established [in the paper] between zeta-potential and particle-particle attraction could be potentially applied to self-assembling proteins in general."

When asked why he selected Malvern's Zetasizer Nano for his work, Dr Uskokovic said: "I had previous experience with the Zetasizer Nano, albeit with more robust inorganic substances, so when asked to purchase a protein particle characterization system I knew it might be a good candidate. In a side by side trial with a competitive product, there was no contest. Results from the Zetasizer Nano were both more reliable and more robust."

Dr Uskokovic continued: "Proteins are sensitive to electric fields and during zeta potential measurements proteins are known to accidentally ‘cook' if you're not careful. To achieve usable results, it was therefore necessary to fine tune our procedures to overcome these challenges and it was a huge advantage to have the experts at Malvern Instruments so readily available to answer questions and deliver detailed guidance. As a result, I am now able to pass on this tutelage to students volunteering in our lab."

Having completed the initial study covering the characterization of amelogenin protein, including its proteolytic cleavage products, as published in the Journal of Dental Research, Dr Uskokovic is currently awaiting publication of a more comprehensive paper focusing on the interaction between amelogenin and its mineral counterpart in enamel.

Malvern Instruments Zetasizer Nano delivers high quality DLS and zeta potential measurements in a single instrument. The combination of these techniques, and the reliability of results, makes the instrument ideal for protein characterization. For further information, please visit:

Malvern, Malvern Instruments and Zetasizer are registered trademarks of Malvern Instruments Ltd


About Malvern Instruments
Malvern Instruments is a market leader in measuring performance controlling material properties. These include particle size, particle shape, zeta potential, molecular weight, size and conformation, rheological properties and chemical distribution. Malvern delivers the systems, support and expertise that ensure the analytical integrity and productivity needed to drive research, development and manufacturing.

Malvern’s measurement solutions for scientists, technologists and engineers advance continually through customer collaboration. Complementary materials characterization systems deliver inter-related measurements that reflect the complexities of particulates and disperse systems, nanomaterials and macromolecules. Combining intelligently implemented technologies with in-depth industry applications knowledge and support, Malvern provides customers with the competitive advantage they demand.

Headquartered in Malvern, UK, Malvern Instruments has subsidiary organizations in all major European markets, North America, China, Korea and Japan, a joint venture in India, a global distributor network and applications laboratories around the world.

For more information, please click here

For press information, please contact:

Trish Appleton
Kapler Communications
Knowledge Centre
Wyboston Lakes
Great North Road
MK44 3BY
Tel: +44 (0)1480 479280
Fax: +44 (0)1480 470343

USA contact:

Marisa Fraser
Malvern Instruments Inc.
117 Flanders Road
MA 01581-1042
Tel: +1 508 768 6400
Fax: +1 508 768 6403

Please send sales enquiries to:

Alison Vines
Malvern Instruments Ltd
Enigma Business Park
Grovewood Road
WR14 1XZ
Tel: +44 (0) 1684 892456
Fax: +44 (0) 1684 892789

Copyright © Malvern Instruments

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Get ready for NanoDays! March 5th, 2015

American Chemical Society Presidential Symposia: nanoscience, international chemistry March 5th, 2015

CiQUS researchers obtain high-quality perovskites over large areas by a chemical method March 4th, 2015

Arrowhead to Present at 2015 Barclays Global Healthcare Conference March 4th, 2015


New research could lead to more efficient electrical energy storage March 4th, 2015

Energy-generating cloth could replace batteries in wearable devices March 4th, 2015

The taming of magnetic vortices: Unified theory for skyrmion-materials March 3rd, 2015

Black phosphorus is new 'wonder material' for improving optical communication March 3rd, 2015

Self Assembly

Nanotubes self-organize and wiggle: Evolution of a nonequilibrium system demonstrates MEPP February 10th, 2015

Engineering self-assembling amyloid fibers January 26th, 2015

Revealed: How bacteria drill into our cells and kill them December 2nd, 2014

Live Images from the Nano-cosmos: Researchers watch layers of football molecules grow November 5th, 2014


Get ready for NanoDays! March 5th, 2015

American Chemical Society Presidential Symposia: nanoscience, international chemistry March 5th, 2015

CiQUS researchers obtain high-quality perovskites over large areas by a chemical method March 4th, 2015

Arrowhead to Present at 2015 Barclays Global Healthcare Conference March 4th, 2015


Keysight Technologies Shifts to Direct Sales of High-Performance Products in North America March 3rd, 2015

Forbidden quantum leaps possible with high-res spectroscopy March 2nd, 2015

International research partnership tricks the light fantastic March 2nd, 2015

Important step towards quantum computing: Metals at atomic scale March 2nd, 2015


A potential long-lasting treatment for sensitive teeth January 7th, 2015

Nanocomposites Strengthen Bone Implants November 13th, 2014

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

Nanoparticles Used to Improve Quality of Bone Cement September 29th, 2014

The latest news from around the world, FREE

  Premium Products
Only the news you want to read!
 Learn More
University Technology Transfer & Patents
 Learn More
Full-service, expert consulting
 Learn More

Nanotechnology Now Featured Books


The Hunger Project

© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE