Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Researchers show how cells open 'doors' to release neurotransmitters

A schematic model of a fusion pore opening.
A schematic model of a fusion pore opening.

Abstract:
Like opening a door to exit a room, cells in the body open up their outer membranes to release such chemicals as neurotransmitters and other hormones.

Cornell researchers have shed new light on this lightning-quick, impossibly small-scale process, called exocytosis, by casting sharp focus on what happens right at the moment the "doors" on the cell wall open.

By Anne Ju

Researchers show how cells open 'doors' to release neurotransmitters

Ithaca, NY | Posted on October 13th, 2010

Publishing online Oct. 11 in Proceedings of the National Academy of Sciences, researchers led by Cornell's Manfred Lindau used a combination of molecular biology, electrophysiology, microfabricated electrochemical sensors and advanced microscopy to elucidate exocytosis of noradrenaline. This is a neurotransmitter released from the adrenal gland by a type of neuroendocrine cell called a chromaffin cell.

Lindau, professor of applied and engineering physics, studies the properties of exocytosis by looking at how packets of chemicals called vesicles adhere to the cell wall and open the door between the vesicle interior and cell's exterior. This "door" is called the fusion pore.

"Biochemists have been working on experiments to identify what proteins and molecules are the main players in this mechanism of release," Lindau said.

It turns out that neurotransmitter release is largely regulated by a set of proteins called SNARE proteins, and one called synaptobrevin is located on the cell's vesicle membrane. The synaptobrevins bind with other proteins called syntaxin and SNAP-25, which are located in the plasma membrane that encloses the cell. When the cell is excited and the neurotransmitter release is triggered, these proteins together are believed to open the fusion pore.

Lindau's team of researchers used genetically altered mouse embryos that lacked synaptobrevin and introduced viruses with modified versions of the protein into their experiments. They then imaged and studied the release function of the cells for the different versions of the synaptobrevins.

They discovered that one end of the synaptobrevin -- the part that anchors it in the vesicle membrane -- is pulled deeper into the vesicle membrane when the cell is stimulated. This movement is what temporarily changes the structure of the membrane and allows the opening of the fusion pore and neurotransmitter release. It had previously been thought that the fusion pore originates by an indirect effect of the SNARE protein in the membrane lipids. Lindau's experiments have shown that the vesicle membrane component of synaptobrevin is the active part of the molecular nanomachine that forms the fusion pore.

To continue visualizing the molecular details of this complex process, Lindau is working on sabbatical at the University of Oxford with professor Mark Sansom on molecular dynamics and computer simulations of the SNARE proteins and neurotransmitter exocytosis.

The research published in PNAS was funded primarily by the National Institutes of Health and the Cornell Nanobiotechnology Center, which is supported by the National Science Foundation. The paper's authors include former Cornell graduate students Annita Ngatchou and Kassandra Kisler, postdoctoral associates Qinghua Fang and Yong Zhao, and collaborators from the Max Planck Institute for Biophysical Chemistry, the University of Saarland in Germany and the University of Copenhagen.

####

For more information, please click here

Contacts:
Media Contact:
Joe Schwartz
(607) 254-6235


Cornell Chronicle:
Anne Ju
(607) 255-9735

Copyright © Cornell University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Measuring the Smallest Magnets July 28th, 2014

WITec to host the 11th Confocal Raman Imaging Symposium from September 29th - October 1st in Ulm, Germany July 28th, 2014

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

Production of Toxic Gas Sensor Based on Nanorods July 28th, 2014

Govt.-Legislation/Regulation/Funding/Policy

Seeing is bead-lieving: Rice University scientists create model 'bead-spring' chains with tunable properties July 28th, 2014

Stanford team achieves 'holy grail' of battery design: A stable lithium anode - Engineers use carbon nanospheres to protect lithium from the reactive and expansive problems that have restricted its use as an anode July 27th, 2014

New imaging agent provides better picture of the gut July 25th, 2014

A*STAR and industry form S$200M semiconductor R&D July 25th, 2014

Academic/Education

Haydale Announces Collaboration Agreement with Swansea University’s Welsh Centre for Printing and Coatings (WCPC) July 12th, 2014

STFC takes delivery of the 100th Hitachi Tabletop SEM in the UK July 3rd, 2014

Innovation Management and the Emergence of the Nanobiotechnology Industry July 1st, 2014

Albany NanoCollege Faculty Member Selected as Editor-in-Chief of the Prestigious Journal of Electronic Materials July 1st, 2014

Molecular Machines

Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster: University of Leicester research team unlocks insights into creation of new nano-materials July 25th, 2014

NIST shows ultrasonically propelled nanorods spin dizzyingly fast July 22nd, 2014

University of Illinois researchers demonstrate novel, tunable nanoantennas July 14th, 2014

Ribosome Research in Atomic Detail Offers Potential Insights into Cancer, Anemia, Alzheimer’s: New movement during decoding occurs in humans, not in bacteria July 3rd, 2014

Nanomedicine

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

New imaging agent provides better picture of the gut July 25th, 2014

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

Researchers create vaccine for dust-mite allergies Main Page Content: Vaccine reduced lung inflammation to allergens in lab and animal tests July 22nd, 2014

Announcements

Measuring the Smallest Magnets July 28th, 2014

WITec to host the 11th Confocal Raman Imaging Symposium from September 29th - October 1st in Ulm, Germany July 28th, 2014

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

Production of Toxic Gas Sensor Based on Nanorods July 28th, 2014

Nanobiotechnology

Seeing is bead-lieving: Rice University scientists create model 'bead-spring' chains with tunable properties July 28th, 2014

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

Production of Non-Virus Nanocarriers with Highest Amount of Gene Delivery July 17th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE