Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Catalyst Sandwich

Chad Mirkin
Chad Mirkin

Abstract:
Synthetic PCR mimic could lead to highly sensitive medical, environmental diagnostics

By Megan Fellman

Catalyst Sandwich

Evanston, IL | Posted on October 7th, 2010

Northwestern University researchers have taken another step towards realizing a new class of polymerase chain reaction (PCR) enzyme mimics, opening the door for the development of highly sensitive chemical detection systems that go beyond nucleic acid targets.

The blueprint for building synthetic structures to detect and signal the presence of targets such as small molecule medical analytes (signalers of disease or bodily malfunction, such as neurotransmitters) and environmental hazards, such as TNT, to name just a few, is inspired by biology and its allosteric enzymes. The method also could be useful in catalysis and the production of polymers, including plastics.

The work, which promises higher sensitivity than that of current detection tools, will be published Oct. 1 by the journal Science.

"PCR -- the backbone of the biodiagnostics industry -- is an enzyme that binds to a nucleic acid and changes shape, turning on a catalyst that makes copies of the nucleic acid for detection purposes," said Chad A. Mirkin, George B. Rathmann Professor of Chemistry in the Weinberg College of Arts and Sciences.

"What if you could do that for thousands of small molecules of interest?" he said. "We'd like to be able to detect tiny amounts of targets important to medicine and the environment, opening avenues to new types of diagnostic tools, just as PCR did for the modern fields of medical diagnostics and forensics. Our new catalysts could make that possible."

Mirkin led a team of chemists who built a synthetic structure that, much like the layers of an Oreo cookie, sandwiches the catalyst between two chemically inert layers. This triple-layer architecture allows the use of any catalyst as it will be kept inactive, or in an "off" state, until triggered by a specific small molecule.

The enzyme mimic behaves like allosteric enzymes found in nature, catalysts that change shape to carry out their functions. (Hemoglobin is an example of an allosteric enzyme.) When the mimic reacts with a specific small molecule, the triple-layer structure changes shape and opens, exposing the catalyst. The resulting catalytic reaction signals the presence of the small molecule target, much like PCR amplifies a single piece of DNA.

"One of our challenges as synthetic chemists has been learning to synthesize structures inspired by biology but that have nothing to do with biology other than the fact we'd like such complex functions realized in man-made systems," said Mirkin, also director of Northwestern's International Institute for Nanotechnology.

In the work reported in Science, the researchers use an aluminum salen complex as the catalyst in the three-layer structure. The addition of chloride (the reduced form of chlorine) triggers the catalyst and starts the polymerization process. (Chloride ion binds at an allosteric binding site, distant from the active or catalytic site.) The addition of an agent that removes the chloride stops the process, but the chloride can be added back to start it again.

The U.S. Air Force of Scientific Research, the Army Research Office and the National Science Foundation supported the research.

The title of the paper is "Allosteric Supramolecular Triple-Layer Catalysts." In addition to Mirkin, other authors of the paper are Hyo Jae Yoon, Junpei Kuwabara and Jun-Hyun Kim, all from Northwestern.

####

For more information, please click here

Contacts:
Megan Fellman

Copyright © Northwestern University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Iranian Researchers Planning to Produce Edible Insulin January 28th, 2015

Nanoparticles that deliver oligonucleotide drugs into cells described in Nucleic Acid Therapeutics January 28th, 2015

'Bulletproof' battery: Kevlar membrane for safer, thinner lithium rechargeables January 28th, 2015

Spider electro-combs its sticky nano-filaments January 28th, 2015

Govt.-Legislation/Regulation/Funding/Policy

Nanoscale Mirrored Cavities Amplify, Connect Quantum Memories: Advance could lead to quantum computing and the secure transfer of information over long-distance fiber optic networks January 28th, 2015

Detecting chemical weapons with a color-changing film January 28th, 2015

'Bulletproof' battery: Kevlar membrane for safer, thinner lithium rechargeables January 28th, 2015

Researchers Make Magnetic Graphene: UC Riverside research could lead to new multi-functional electronic devices January 27th, 2015

Possible Futures

GS7 Graphene Sensor maybe Solution in Fight Against Cancer January 25th, 2015

Nanotechnology in Energy Applications Market Research Report 2014-2018: Radiant Insights, Inc January 15th, 2015

'Mind the gap' between atomically thin materials December 23rd, 2014

A novel method for identifying the body’s ‘noisiest’ networks November 19th, 2014

Academic/Education

Rice's Naomi Halas to direct Smalley Institute: Optics pioneer will lead Rice's multidisciplinary science institute January 15th, 2015

SUNY Board Appoints Dr. Alain Kaloyeros as Founding President of SUNY Polytechnic Institute January 13th, 2015

CNSE's Smart System Technology & Commercialization Center Successfully Recertifies as ISO 9001:2008 January 12th, 2015

SUNY Poly Now Accepting Applications to the Colleges of Nanoscale Science and Engineering for Fall 2015: Full Scholarships Available to Incoming CNSE Students January 7th, 2015

Nanomedicine

Iranian Researchers Planning to Produce Edible Insulin January 28th, 2015

Nanoparticles that deliver oligonucleotide drugs into cells described in Nucleic Acid Therapeutics January 28th, 2015

Nanoliposomes Help Efforts to Cure Bacterial Infections January 27th, 2015

Stomach acid-powered micromotors get their first test in a living animal January 27th, 2015

Announcements

Iranian Researchers Planning to Produce Edible Insulin January 28th, 2015

Nanoparticles that deliver oligonucleotide drugs into cells described in Nucleic Acid Therapeutics January 28th, 2015

'Bulletproof' battery: Kevlar membrane for safer, thinner lithium rechargeables January 28th, 2015

Spider electro-combs its sticky nano-filaments January 28th, 2015

Environment

Detection of Heavy Metals in Samples with Naked Eye January 26th, 2015

Magnetic Nanosorbents Able to Eliminate Chemical Contaminants January 19th, 2015

Malaysian Nanotechnology Company Nanopac Innovation Ltd. lists on the NSX January 19th, 2015

Iran Designs Magnetic Nano-Absorbents Cleaning Chemical Pollutants January 11th, 2015

Nanobiotechnology

Spider electro-combs its sticky nano-filaments January 28th, 2015

Nanoshuttle wear and tear: It's the mileage, not the age January 26th, 2015

Engineering self-assembling amyloid fibers January 26th, 2015

DNA 'glue' could someday be used to build tissues, organs January 14th, 2015

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE