Nanotechnology Now





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Catalyst Sandwich

Chad Mirkin
Chad Mirkin

Abstract:
Synthetic PCR mimic could lead to highly sensitive medical, environmental diagnostics

By Megan Fellman

Catalyst Sandwich

Evanston, IL | Posted on October 7th, 2010

Northwestern University researchers have taken another step towards realizing a new class of polymerase chain reaction (PCR) enzyme mimics, opening the door for the development of highly sensitive chemical detection systems that go beyond nucleic acid targets.

The blueprint for building synthetic structures to detect and signal the presence of targets such as small molecule medical analytes (signalers of disease or bodily malfunction, such as neurotransmitters) and environmental hazards, such as TNT, to name just a few, is inspired by biology and its allosteric enzymes. The method also could be useful in catalysis and the production of polymers, including plastics.

The work, which promises higher sensitivity than that of current detection tools, will be published Oct. 1 by the journal Science.

"PCR -- the backbone of the biodiagnostics industry -- is an enzyme that binds to a nucleic acid and changes shape, turning on a catalyst that makes copies of the nucleic acid for detection purposes," said Chad A. Mirkin, George B. Rathmann Professor of Chemistry in the Weinberg College of Arts and Sciences.

"What if you could do that for thousands of small molecules of interest?" he said. "We'd like to be able to detect tiny amounts of targets important to medicine and the environment, opening avenues to new types of diagnostic tools, just as PCR did for the modern fields of medical diagnostics and forensics. Our new catalysts could make that possible."

Mirkin led a team of chemists who built a synthetic structure that, much like the layers of an Oreo cookie, sandwiches the catalyst between two chemically inert layers. This triple-layer architecture allows the use of any catalyst as it will be kept inactive, or in an "off" state, until triggered by a specific small molecule.

The enzyme mimic behaves like allosteric enzymes found in nature, catalysts that change shape to carry out their functions. (Hemoglobin is an example of an allosteric enzyme.) When the mimic reacts with a specific small molecule, the triple-layer structure changes shape and opens, exposing the catalyst. The resulting catalytic reaction signals the presence of the small molecule target, much like PCR amplifies a single piece of DNA.

"One of our challenges as synthetic chemists has been learning to synthesize structures inspired by biology but that have nothing to do with biology other than the fact we'd like such complex functions realized in man-made systems," said Mirkin, also director of Northwestern's International Institute for Nanotechnology.

In the work reported in Science, the researchers use an aluminum salen complex as the catalyst in the three-layer structure. The addition of chloride (the reduced form of chlorine) triggers the catalyst and starts the polymerization process. (Chloride ion binds at an allosteric binding site, distant from the active or catalytic site.) The addition of an agent that removes the chloride stops the process, but the chloride can be added back to start it again.

The U.S. Air Force of Scientific Research, the Army Research Office and the National Science Foundation supported the research.

The title of the paper is "Allosteric Supramolecular Triple-Layer Catalysts." In addition to Mirkin, other authors of the paper are Hyo Jae Yoon, Junpei Kuwabara and Jun-Hyun Kim, all from Northwestern.

####

For more information, please click here

Contacts:
Megan Fellman

Copyright © Northwestern University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Researchers find the 'key' to quantum network solution May 25th, 2015

One step closer to a single-molecule device: Columbia Engineering researchers first to create a single-molecule diode -- the ultimate in miniaturization for electronic devices -- with potential for real-world applications May 25th, 2015

DNA Double Helix Does Double Duty in Assembling Arrays of Nanoparticles: Synthetic pieces of biological molecule form framework and glue for making nanoparticle clusters and arrays May 25th, 2015

Engineering Phase Changes in Nanoparticle Arrays: Scientists alter attractive and repulsive forces between DNA-linked particles to make dynamic, phase-shifting forms of nanomaterials May 25th, 2015

Govt.-Legislation/Regulation/Funding/Policy

Researchers find the 'key' to quantum network solution May 25th, 2015

One step closer to a single-molecule device: Columbia Engineering researchers first to create a single-molecule diode -- the ultimate in miniaturization for electronic devices -- with potential for real-world applications May 25th, 2015

DNA Double Helix Does Double Duty in Assembling Arrays of Nanoparticles: Synthetic pieces of biological molecule form framework and glue for making nanoparticle clusters and arrays May 25th, 2015

Engineering Phase Changes in Nanoparticle Arrays: Scientists alter attractive and repulsive forces between DNA-linked particles to make dynamic, phase-shifting forms of nanomaterials May 25th, 2015

Possible Futures

Simulations predict flat liquid May 21st, 2015

Nature inspires first artificial molecular pump: Simple design mimics pumping mechanism of life-sustaining proteins found in living cells May 19th, 2015

NNCO and Museum of Science Fiction to Collaborate on Nanotechnology and 3D Printing Panels at Awesome Con May 19th, 2015

Quantum 'gruyres' for spintronics of the future: Topological insulators become a little less 'elusive' May 12th, 2015

Academic/Education

SUNY Poly CNSE and NIOSH Launch Federal Nano Health and Safety Consortium: May 20th, 2015

New JEOL E-Beam Lithography System to Enhance Quantum NanoFab Capabilities May 6th, 2015

FEI Partners With the George Washington University to Equip New Science & Engineering Hall: Suite of new high-performance microscopes will be used for cutting-edge experiments at GWs new research facility April 29th, 2015

Renishaw Raman systems used to study 2D materials at Boston University, Massachusetts, USA. April 28th, 2015

Nanomedicine

DNA Double Helix Does Double Duty in Assembling Arrays of Nanoparticles: Synthetic pieces of biological molecule form framework and glue for making nanoparticle clusters and arrays May 25th, 2015

Nanostructures Increase Corrosion Resistance in Metallic Body Implants May 24th, 2015

Iranian Scientists Use Magnetic Field to Transfer Anticancer Drug to Tumor Tissue May 24th, 2015

New Antibacterial Wound Dressing in Iran Can Display Replacement Time May 22nd, 2015

Announcements

Researchers find the 'key' to quantum network solution May 25th, 2015

One step closer to a single-molecule device: Columbia Engineering researchers first to create a single-molecule diode -- the ultimate in miniaturization for electronic devices -- with potential for real-world applications May 25th, 2015

DNA Double Helix Does Double Duty in Assembling Arrays of Nanoparticles: Synthetic pieces of biological molecule form framework and glue for making nanoparticle clusters and arrays May 25th, 2015

Engineering Phase Changes in Nanoparticle Arrays: Scientists alter attractive and repulsive forces between DNA-linked particles to make dynamic, phase-shifting forms of nanomaterials May 25th, 2015

Environment

Conversion of Greenhouse Gases to Syngas in Presence of Nanocatalysts in Iran May 22nd, 2015

Directa Plus in Barcelona to present the innovative project GEnIuS for oil spills clean-up activities: The company has created a graphene-based product for the remediation of water contaminated by oil and hydrocarbons May 21st, 2015

Nano-policing pollution May 13th, 2015

Chemists strike nano-gold: 4 new atomic structures for gold nanoparticle clusters: Research builds upon work by Nobel Prize-winning team from Stanford University April 28th, 2015

Nanobiotechnology

DNA Double Helix Does Double Duty in Assembling Arrays of Nanoparticles: Synthetic pieces of biological molecule form framework and glue for making nanoparticle clusters and arrays May 25th, 2015

Engineering Phase Changes in Nanoparticle Arrays: Scientists alter attractive and repulsive forces between DNA-linked particles to make dynamic, phase-shifting forms of nanomaterials May 25th, 2015

This Slinky lookalike 'hyperlens' helps us see tiny objects: The photonics advancement could improve early cancer detection, nanoelectronics manufacturing and scientists' ability to observe single molecules May 23rd, 2015

Supercomputer unlocks secrets of plant cells to pave the way for more resilient crops: IBM partners with University of Melbourne and UQ May 21st, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project