Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > A new use for gold

An image of gold nanoparticles. Image courtesy Kimberly Hamad-Schifferli
An image of gold nanoparticles. Image courtesy Kimberly Hamad-Schifferli

Abstract:
Engineers turn a drawback — the stickiness of gold nanoparticles — into an advantage.

By Diana LaScala-Gruenewald, MIT News correspondent

A new use for gold

Cambridge, MA | Posted on June 11th, 2010

Gold nanoparticles — tiny spheres of gold just a few billionths of a meter in diameter — have become useful tools in modern medicine. They've been incorporated into miniature drug-delivery systems to control blood clotting, and they're also the main components of a device, now in clinical trials, that is designed to burn away malignant tumors.

However, one property of these particles stands in the way of many nanotechnological developments: They‘re sticky. Gold nanoparticles can be engineered to attract specific biomolecules, but they also stick to many other unintended particles — often making them inefficient at their designated task.

MIT researchers have found a way to turn this drawback into an advantage. In a paper recently published in American Chemical Society Nano, Associate Professor Kimberly Hamad-Schifferli of the Departments of Biological Engineering and Mechanical Engineering and postdoc Sunho Park PhD '09 of the Department of Mechanical Engineering reported that they could exploit nanoparticles' stickiness to double the amount of protein produced during in vitro translation — an important tool that biologists use to safely produce a large quantity of protein for study outside of a living cell.

During translation, groups of biomolecules come together to produce proteins from molecular templates called mRNA. In vitro translation harnesses these same biological components in a test tube (as opposed to in vivo translation, which occurs in live cells), and a man-made mRNA can be added to guarantee the production of a desired protein. For example, if a researcher wanted to study a protein that a cell would not naturally produce, or a mutated protein that would be harmful to the cell in vivo, he might use in vitro translation to create large quantities of that protein for observation and testing. But there's a downside to in vitro translation: It is not as efficient as it could be. "You might get some protein one day, and none for the next two," explains Hamad-Schifferli.

With funding from the Institute of Biomedical Imaging and Bioengineering, Hamad-Schifferli and her co-workers initially set out to design a system that would prevent translation. This process, known as translation inhibition, can stop the production of harmful proteins or help a researcher determine protein function by observing cell behavior when the protein has been removed. To accomplish this, Hamad-Schifferli attached DNA to gold nanoparticles, expecting that the large nanoparticle-DNA (NP-DNA) aggregates would block translation.

She was discouraged, however, to find that the NP-DNA did not decrease protein production as expected. In fact, she had some unsettling data suggesting that instead of inhibiting translation, the NP-DNA were boosting it. "That's when we put on our engineering caps," recalls Hamad-Schifferli.

It turns out that the sticky nanoparticles bring the biomolecules needed for translation into close proximity, which helps speed the translation process. Additionally, the DNA part of the NP-DNA complex is designed to bind to a specific mRNA molecule, which will be translated into a specific protein. The binding must be tight enough to hold the mRNA in place for translation, but loose enough that the mRNA can also attach to the other molecules necessary for the process. Because the designed DNA molecule has a specific mRNA partner, that mRNA in a solution of many similar molecules can be enhanced without having to be isolated.

In addition to enhancing in vitro translation, Hamad-Schifferli's NP-DNA complexes may have other applications. According to Ming Zheng, a research chemist with the National Institute of Standards and Technology, they could be combined with carbon nanotubes — tiny, hollow cylinders that are incredibly strong for their size. They may ultimately be the cornerstone of transport systems that ferry drugs into cells or between cells. The stickiness of the NP-DNA might enhance the speed and accuracy of such a drug-delivery system.

Although Hamad-Schifferli is confident that her discovery will make in vitro translation more reliable and efficient, she is not done. She hopes to tinker with her system to further enhance protein production in vitro, and see if the system can be applied to enhance translation in live cells. To help reach these goals, she must design and conduct experiments to determine which molecules are involved in the enhancement process, and how they interact. "The upside is that we've been lucky," Hamad-Schifferli says, reflecting on her discovery. "The downside is that it will be difficult to tease out exactly how the system works."

####

For more information, please click here

Copyright © MIT

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

A step closer to understanding quantum mechanics: Swansea University’s physicists develop a new quantum simulation protocol October 22nd, 2017

Creation of coherent states in molecules by incoherent electrons October 21st, 2017

Strange but true: turning a material upside down can sometimes make it softer October 20th, 2017

Leti Coordinating Project to Develop Innovative Drivetrains for 3rd-generation Electric Vehicles: CEA Tech’s Contribution Includes Liten’s Knowhow in Magnetic Materials and Simulation And Leti’s Expertise in Wide-bandgap Semiconductors October 20th, 2017

Govt.-Legislation/Regulation/Funding/Policy

Leti Coordinating Project to Develop Innovative Drivetrains for 3rd-generation Electric Vehicles: CEA Tech’s Contribution Includes Liten’s Knowhow in Magnetic Materials and Simulation And Leti’s Expertise in Wide-bandgap Semiconductors October 20th, 2017

Bringing the atomic world into full color: Researchers turn atomic force microscope measurements into color images October 19th, 2017

Long nanotubes make strong fibers: Rice University researchers advance characterization, purification of nanotube wires and films October 17th, 2017

Spinning strands hint at folding dynamics: Rice University lab uses magnetic beads to model microscopic proteins, polymers October 17th, 2017

Academic/Education

Two Scientists Receive Grants to Develop New Materials: Chad Mirkin and Monica Olvera de la Cruz recognized by Sherman Fairchild Foundation August 16th, 2017

Moving at the Speed of Light: University of Arizona selected for high-impact, industrial demonstration of new integrated photonic cryogenic datalink for focal plane arrays: Program is major milestone for AIM Photonics August 10th, 2017

Graduate Students from Across the Country Attend Hands-on NanoCamp: Prominent scientists Warren Oliver, Ph.D., and George Pharr, Ph.D., presented a weeklong NanoCamp for hand-picked graduate students across the United States July 26th, 2017

The Physics Department of Imperial College, London, uses the Quorum Q150T to deposit metals and ITO to make plasmonic sensors and electric contact pads July 13th, 2017

Nanotubes/Buckyballs/Fullerenes/Nanorods

Long nanotubes make strong fibers: Rice University researchers advance characterization, purification of nanotube wires and films October 17th, 2017

How to draw electricity from the bloodstream: A one-dimensional fluidic nanogenerator with a high power-conversion efficiency September 11th, 2017

Silk could improve sensitivity, flexibility of wearable body sensors August 20th, 2017

Regulation of two-dimensional nanomaterials: New driving force for lithium-ion batteries July 26th, 2017

Nanomedicine

Spinning strands hint at folding dynamics: Rice University lab uses magnetic beads to model microscopic proteins, polymers October 17th, 2017

Arrowhead Pharmaceuticals to Present Preclinical Data on ARO-AAT at The Liver Meeting(R) October 10th, 2017

Arrowhead to Present at Chardan Gene Therapy Conference October 3rd, 2017

'CRISPR-Gold' fixes Duchenne muscular dystrophy mutation in mice October 3rd, 2017

Announcements

A step closer to understanding quantum mechanics: Swansea University’s physicists develop a new quantum simulation protocol October 22nd, 2017

Creation of coherent states in molecules by incoherent electrons October 21st, 2017

Strange but true: turning a material upside down can sometimes make it softer October 20th, 2017

Leti Coordinating Project to Develop Innovative Drivetrains for 3rd-generation Electric Vehicles: CEA Tech’s Contribution Includes Liten’s Knowhow in Magnetic Materials and Simulation And Leti’s Expertise in Wide-bandgap Semiconductors October 20th, 2017

Nanobiotechnology

Spinning strands hint at folding dynamics: Rice University lab uses magnetic beads to model microscopic proteins, polymers October 17th, 2017

Arrowhead Pharmaceuticals to Present Preclinical Data on ARO-AAT at The Liver Meeting(R) October 10th, 2017

Arrowhead to Present at Chardan Gene Therapy Conference October 3rd, 2017

'CRISPR-Gold' fixes Duchenne muscular dystrophy mutation in mice October 3rd, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project