Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > High sensitivity of Zetasizer Nano proves essential for protein aggregation studies at University of South Florida

Abstract:
Dr Martin Muschol, Assistant Professor at the University of South Florida's (USF) Department of Physics and his team are carrying out important research to gain a better understanding of protein aggregation phenomena, including protein crystallization and amyloid fibril growth. Using the Zetasizer Nano ZS, from Malvern Instruments (Malvern, UK), the USF researchers have
been able to perform temperature controlled static and dynamic light scattering (DLS) measurements of protein hydration, protein interactions and aggregation kinetics in vitro.

High sensitivity of Zetasizer Nano proves essential for protein aggregation studies at University of South Florida

Malvern, UK | Posted on April 15th, 2010

"By enabling temperature controlled measurements, the Zetasizer Nano ZS is particularly suited to our work here at USF," said Dr Muschol. "With it we have been able to monitor the effect on the hydration of proteins by salt ions in solution, measuring the hydrodynamic radius of proteins down to plus/minus one tenth the diameter of a water molecule. Interestingly we found that neither chaotropic or cosmotropic salt ions affected overall protein hydration up to salt concentrations of 1M." (ref: ‘Hydration and Hydrodynamic Interactions of Lysozyme: Effects of Chaotropic versus Kosmotropic Ions', Biophysical Journal, Volume 96, pp 3781-3790

Having published several papers in the past year alone, data gathered by Dr Muschol and his team will be used to develop models for protein fibril formation, a behaviour thought to trigger degenerative diseases, such as Alzheimer's disease or Parkinson's disease. "Despite intensive research efforts, we still don't understand how proteins can stay in solution in a crowded cellular environment and what triggers their aggregation and subsequent pathological changes. We hope our research will contribute to resolving these basic puzzles." (ref: ‘Amyloid Protofibrils of Lysozyme Nucleate and Grow Via Oligomer Fusion', Biophysical Journal, Volume 97, pp 590-598)

Dr Muschol's team has also had noticeable success in using the Zetasizer Nano ZS to study the nucleation of gold nanoparticles (ref: R. Jagannathan, et al. J. Phys. Chem. C, 2009, 113, pp3478 3486). Unlike static light measurements commonly used to detect nucleation events, DLS separates out the background scattering from small molecular species in gold sols. Therefore, DLS was able to detect nucleation at a significantly earlier stage than has been possible with other approaches.

The Zetasizer Nano ZS is proving to be the ultimate sizing solution for biotechnology and materials scientists, offering exceptional sensitivity down to sub-micron and nanometre scales. The system's ability to control temperature is important when following the behaviour of polymers and protein samples. Further information can be found at www.malvern.com/zetasizer

Malvern, Malvern Instruments and Zetasizer are registered trademarks of Malvern Instruments Ltd

####

About Malvern Instruments
Malvern Instruments provides a range of complementary materials characterization tools that deliver inter-related measurements reflecting the complexities of particulates and disperse systems, nanomaterials and macromolecules. Analytical instruments from Malvern are used in the characterization of a wide variety of materials, from industrial bulk powders to nanomaterials and delicate macromolecules.

A broad portfolio of innovative technologies is combined with intelligent, user-friendly software. These systems deliver industrially relevant data enabling our customers to make the connection between micro (such as particle size) and macro (bulk) material properties (rheology) and chemical composition (chemical imaging).

Particle size, particle shape, zeta potential, molecular weight, chemical composition and rheological properties measurements are now joined by advanced chromatography solutions (GPC/SEC), extending Malvern’s technologies for protein molecular weight, size and aggregation measurements, and synthetic polymer molecular weight and distribution. The company’s laboratory, at-line, on-line and in-line solutions are proven in sectors as diverse as cement production and pharmaceutical drug discovery.

Headquartered in Malvern, UK, Malvern Instruments has subsidiary organizations in all major European markets, North America, China, Korea and Japan, a joint venture in India, a global distributor network and applications laboratories around the world.

For more information, please click here

Contacts:
For press information, please contact:

Trish Appleton
Kapler Communications
Knowledge Centre
Wyboston Lakes
Great North Road
Wyboston
Bedfordshire
MK44 3BY
UK
Tel: +44 (0)1480 479280
Fax: +44 (0)1480 470343

USA contact:

Marisa Fraser
Malvern Instruments Inc.
117 Flanders Road
Westborough
MA 01581-1042
USA
Tel: +1 508 768 6400
Fax: +1 508 768 6403


Please send sales enquiries to:

Alison Vines
Malvern Instruments Ltd
Enigma Business Park
Grovewood Road
Malvern
Worcestershire
WR14 1XZ
UK
Tel: +44 (0) 1684 892456
Fax: +44 (0) 1684 892789

Copyright © Malvern Instruments

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Nano-supercapacitors for electric cars July 25th, 2014

New imaging agent provides better picture of the gut July 25th, 2014

Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster: University of Leicester research team unlocks insights into creation of new nano-materials July 25th, 2014

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

Possible Futures

IBM Announces $3 Billion Research Initiative to Tackle Chip Grand Challenges for Cloud and Big Data Systems: Scientists and engineers to push limits of silicon technology to 7 nanometers and below and create post-silicon future July 10th, 2014

Virus structure inspires novel understanding of onion-like carbon nanoparticles April 10th, 2014

Local girl does good March 22nd, 2014

Surface Characteristics Influence Cellular Growth on Semiconductor Material March 12th, 2014

Academic/Education

Haydale Announces Collaboration Agreement with Swansea University’s Welsh Centre for Printing and Coatings (WCPC) July 12th, 2014

STFC takes delivery of the 100th Hitachi Tabletop SEM in the UK July 3rd, 2014

Innovation Management and the Emergence of the Nanobiotechnology Industry July 1st, 2014

Albany NanoCollege Faculty Member Selected as Editor-in-Chief of the Prestigious Journal of Electronic Materials July 1st, 2014

Nanomedicine

New imaging agent provides better picture of the gut July 25th, 2014

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

Researchers create vaccine for dust-mite allergies Main Page Content: Vaccine reduced lung inflammation to allergens in lab and animal tests July 22nd, 2014

NIST shows ultrasonically propelled nanorods spin dizzyingly fast July 22nd, 2014

Announcements

Nano-supercapacitors for electric cars July 25th, 2014

New imaging agent provides better picture of the gut July 25th, 2014

Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster: University of Leicester research team unlocks insights into creation of new nano-materials July 25th, 2014

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

Tools

Malvern Instruments completes acquisition of MicroCal and announces purchase of Archimedes product from Affinity Biosensors July 25th, 2014

Hysitron is Awarded TWO R&D 100 Awards for Highly Innovative Technology Developments in the Areas of Extreme Environments and Biological Mechanical Property Testing July 23rd, 2014

The Hiden EQP Plasma Diagnostic with on-board MCA July 22nd, 2014

Nanometrics Announces Upcoming Investor Events July 22nd, 2014

Nanobiotechnology

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

Production of Non-Virus Nanocarriers with Highest Amount of Gene Delivery July 17th, 2014

Physicists Use Computer Models to Reveal Quantum Effects in Biological Oxygen Transport: The team solved a long-standing question by explaining why oxygen – and not deadly carbon monoxide – preferably binds to the proteins that transport it around the body. July 17th, 2014

Tiny DNA pyramids enter bacteria easily -- and deliver a deadly payload July 9th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE