Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > High sensitivity of Zetasizer Nano proves essential for protein aggregation studies at University of South Florida

Abstract:
Dr Martin Muschol, Assistant Professor at the University of South Florida's (USF) Department of Physics and his team are carrying out important research to gain a better understanding of protein aggregation phenomena, including protein crystallization and amyloid fibril growth. Using the Zetasizer Nano ZS, from Malvern Instruments (Malvern, UK), the USF researchers have
been able to perform temperature controlled static and dynamic light scattering (DLS) measurements of protein hydration, protein interactions and aggregation kinetics in vitro.

High sensitivity of Zetasizer Nano proves essential for protein aggregation studies at University of South Florida

Malvern, UK | Posted on April 15th, 2010

"By enabling temperature controlled measurements, the Zetasizer Nano ZS is particularly suited to our work here at USF," said Dr Muschol. "With it we have been able to monitor the effect on the hydration of proteins by salt ions in solution, measuring the hydrodynamic radius of proteins down to plus/minus one tenth the diameter of a water molecule. Interestingly we found that neither chaotropic or cosmotropic salt ions affected overall protein hydration up to salt concentrations of 1M." (ref: ‘Hydration and Hydrodynamic Interactions of Lysozyme: Effects of Chaotropic versus Kosmotropic Ions', Biophysical Journal, Volume 96, pp 3781-3790

Having published several papers in the past year alone, data gathered by Dr Muschol and his team will be used to develop models for protein fibril formation, a behaviour thought to trigger degenerative diseases, such as Alzheimer's disease or Parkinson's disease. "Despite intensive research efforts, we still don't understand how proteins can stay in solution in a crowded cellular environment and what triggers their aggregation and subsequent pathological changes. We hope our research will contribute to resolving these basic puzzles." (ref: ‘Amyloid Protofibrils of Lysozyme Nucleate and Grow Via Oligomer Fusion', Biophysical Journal, Volume 97, pp 590-598)

Dr Muschol's team has also had noticeable success in using the Zetasizer Nano ZS to study the nucleation of gold nanoparticles (ref: R. Jagannathan, et al. J. Phys. Chem. C, 2009, 113, pp3478 3486). Unlike static light measurements commonly used to detect nucleation events, DLS separates out the background scattering from small molecular species in gold sols. Therefore, DLS was able to detect nucleation at a significantly earlier stage than has been possible with other approaches.

The Zetasizer Nano ZS is proving to be the ultimate sizing solution for biotechnology and materials scientists, offering exceptional sensitivity down to sub-micron and nanometre scales. The system's ability to control temperature is important when following the behaviour of polymers and protein samples. Further information can be found at www.malvern.com/zetasizer

Malvern, Malvern Instruments and Zetasizer are registered trademarks of Malvern Instruments Ltd

####

About Malvern Instruments
Malvern Instruments provides a range of complementary materials characterization tools that deliver inter-related measurements reflecting the complexities of particulates and disperse systems, nanomaterials and macromolecules. Analytical instruments from Malvern are used in the characterization of a wide variety of materials, from industrial bulk powders to nanomaterials and delicate macromolecules.

A broad portfolio of innovative technologies is combined with intelligent, user-friendly software. These systems deliver industrially relevant data enabling our customers to make the connection between micro (such as particle size) and macro (bulk) material properties (rheology) and chemical composition (chemical imaging).

Particle size, particle shape, zeta potential, molecular weight, chemical composition and rheological properties measurements are now joined by advanced chromatography solutions (GPC/SEC), extending Malvern’s technologies for protein molecular weight, size and aggregation measurements, and synthetic polymer molecular weight and distribution. The company’s laboratory, at-line, on-line and in-line solutions are proven in sectors as diverse as cement production and pharmaceutical drug discovery.

Headquartered in Malvern, UK, Malvern Instruments has subsidiary organizations in all major European markets, North America, China, Korea and Japan, a joint venture in India, a global distributor network and applications laboratories around the world.

For more information, please click here

Contacts:
For press information, please contact:

Trish Appleton
Kapler Communications
Knowledge Centre
Wyboston Lakes
Great North Road
Wyboston
Bedfordshire
MK44 3BY
UK
Tel: +44 (0)1480 479280
Fax: +44 (0)1480 470343

USA contact:

Marisa Fraser
Malvern Instruments Inc.
117 Flanders Road
Westborough
MA 01581-1042
USA
Tel: +1 508 768 6400
Fax: +1 508 768 6403


Please send sales enquiries to:

Alison Vines
Malvern Instruments Ltd
Enigma Business Park
Grovewood Road
Malvern
Worcestershire
WR14 1XZ
UK
Tel: +44 (0) 1684 892456
Fax: +44 (0) 1684 892789

Copyright © Malvern Instruments

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

A new product to help combat mouldy walls, thanks to technology developed at the ICN2 December 14th, 2017

Sandia researchers make solid ground toward better lithium-ion battery interfaces: Reducing the traffic jam in batteries December 13th, 2017

Perking up and crimping the 'bristles' of polyelectrolyte brushes December 13th, 2017

Columbia engineers create artificial graphene in a nanofabricated semiconductor structure: Researchers are the first to observe the electronic structure of graphene in an engineered semiconductor; finding could lead to progress in advanced optoelectronics and data processing December 13th, 2017

Possible Futures

Sandia researchers make solid ground toward better lithium-ion battery interfaces: Reducing the traffic jam in batteries December 13th, 2017

Perking up and crimping the 'bristles' of polyelectrolyte brushes December 13th, 2017

Columbia engineers create artificial graphene in a nanofabricated semiconductor structure: Researchers are the first to observe the electronic structure of graphene in an engineered semiconductor; finding could lead to progress in advanced optoelectronics and data processing December 13th, 2017

Untangling DNA: Researchers filter the entropy out of nanopore measurements December 8th, 2017

Academic/Education

Luleå University of Technology is using the Deben CT5000TEC stage to perform x-ray microtomography experiments with the ZEISS Xradia 510 Versa to understand deformation and strain inside inhomogeneous materials November 7th, 2017

Park Systems Announces the Grand Opening of the Park NanoScience Center at SUNY Polytechnic Institute November 3rd, 2017

Two Scientists Receive Grants to Develop New Materials: Chad Mirkin and Monica Olvera de la Cruz recognized by Sherman Fairchild Foundation August 16th, 2017

Moving at the Speed of Light: University of Arizona selected for high-impact, industrial demonstration of new integrated photonic cryogenic datalink for focal plane arrays: Program is major milestone for AIM Photonics August 10th, 2017

Nanomedicine

Perking up and crimping the 'bristles' of polyelectrolyte brushes December 13th, 2017

Leti to Demo Wristband with Embedded Sensors to Diagnose Sleep Apnea: APNEAband, Which Will Be Demonstrated at CES 2018, Also Monitors Mountain Sickness, Dehydration, Dialysis Treatment Response and Epileptic Seizures December 12th, 2017

Untangling DNA: Researchers filter the entropy out of nanopore measurements December 8th, 2017

Arrowhead Presents New Clinical Data Demonstrating a Sustained Host Response in Hepatitis B Patients Following RNAi Therapy — Up to 5.0 log10 reduction in HBsAg observed; data presented at HEP DART 2017 — December 6th, 2017

Announcements

A new product to help combat mouldy walls, thanks to technology developed at the ICN2 December 14th, 2017

Sandia researchers make solid ground toward better lithium-ion battery interfaces: Reducing the traffic jam in batteries December 13th, 2017

Perking up and crimping the 'bristles' of polyelectrolyte brushes December 13th, 2017

Columbia engineers create artificial graphene in a nanofabricated semiconductor structure: Researchers are the first to observe the electronic structure of graphene in an engineered semiconductor; finding could lead to progress in advanced optoelectronics and data processing December 13th, 2017

Tools

Perking up and crimping the 'bristles' of polyelectrolyte brushes December 13th, 2017

Untangling DNA: Researchers filter the entropy out of nanopore measurements December 8th, 2017

JPK Instruments announce partnership with Swiss company, Cytosurge AG. The partnership makes Cytosurge’s FluidFM® technology available on the JPK NanoWizard® AFM platform December 8th, 2017

Researchers advance technique to detect ovarian cancer: Rice, MD Anderson use fluorescent carbon nanotube probes to achieve first in vivo success November 30th, 2017

Nanobiotechnology

Perking up and crimping the 'bristles' of polyelectrolyte brushes December 13th, 2017

Arrowhead Presents New Clinical Data Demonstrating a Sustained Host Response in Hepatitis B Patients Following RNAi Therapy — Up to 5.0 log10 reduction in HBsAg observed; data presented at HEP DART 2017 — December 6th, 2017

Going swimmingly: Biotemplates breakthrough paves way for cheaper nanobots: By using bacterial flagella as a template for silica, researchers have demonstrated an easier way to make propulsion systems for nanoscale swimming robots November 30th, 2017

Drug-delivering nanoparticles seek and destroy elusive cancer stem cells November 28th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project