Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > JEOL Offers First Commercially-Available Thin Film Phase Plate Technology for TEM

Images of frozen-hydrated T4 phage and influenza A virus courtesy of the laboratory of Prof. K. Nagayama, Okazaki Institute for Integrative Bioscience, Okazaki, Japan.
Images of frozen-hydrated T4 phage and influenza A virus courtesy of the laboratory of Prof. K. Nagayama, Okazaki Institute for Integrative Bioscience, Okazaki, Japan.

Abstract:
Multifold Increase in Biological Specimen Imaging Contrast

JEOL Offers First Commercially-Available Thin Film Phase Plate Technology for TEM

Peabody, MA | Posted on April 7th, 2010

The imaging performance of today's generation of Transmission Electron Microscopes (TEMs) is improved dramatically through the use of a novel technique, the thin film phase plate.

JEOL is the only electron microscope supplier to offer commercially-available thin film phase plate technology to its Life Sciences customers, in particular those involved in cryo-electron microscopy and cryo-electron tomography. The phase contrast imaging capability of a phase-plate outfitted JEOL TEM increases specimen contrast by orders of magnitude.

While phase plate technology - invented by Frits Zernike and awarded the Physic Nobel prize in 1953 - is an accepted tool for live-cell imaging with the light microscope (LM), it is a novel approach to imaging with the TEM. Similar to the LM application, the TEM application employs a -phase plate that shifts the phase of only the scattered electrons, resulting in amplitude contrast rather than phase contrast as these electrons interfere with the unscattered electrons in the image plane.

"Nearly perfect phase-contrast specimens, like frozen-hydrated samples, can now be imaged with superior contrast, which greatly benefits studies where the image contrast is a limiting factor, such as single-particle imaging and tomography." says Dr. Jaap Brink, JEOL's TEM Product Manager. Thus, the phase plate imaging technique stands to have a huge impact in not just the academic field, but also in the field of applied sciences, R&D, or every field where cryo-imaging is being used.

Extensive research has led to the further development of phase plate technology at the laboratory of Prof. Kuniaki Nagayama, Director & Professor of Okazaki Institute for Integrative Bioscience in Japan. Prof. Nagayama and his colleagues have illustrated the potential for routine phase contrast TEM imaging of frozen-hydrated macromolecules. Using custom software they have applied the phase plate imaging successfully to cryo-tomography.

JEOL's TEM columns are optimally designed for phase contrast cryo-electron tomography, as they are unsurpassed in the ability to customize the imaging parameters without sacrificing ease of use. Furthermore, developments have led to continuously heated phase plate holders with piezo drives and software for precise positioning of the phase plate and zero loss energy filtering through an in-column Omega filter.

Within the past few months, JEOL USA has seen an increase in orders for its biological TEMs, especially its 200 and 300 keV models. Collaborative partnerships between these researchers and JEOL USA are aimed at maximizing the efficacy of the phase plate technology.

####

About JEOL
JEOL is a world leader in electron optical equipment and instrumentation for high-end scientific and industrial research and development. Core product groups include electron microscopes (SEMs and TEMs), instruments for the semiconductor industry (electron beam lithography and a series of defect review and inspection tools), and analytical instruments including mass spectrometers, NMRs and ESRs.

JEOL USA, Inc., a wholly owned subsidiary of JEOL, Ltd., Japan, was incorporated in the United States in 1962. The company has 13 regional service centers that offer unlimited emergency service and support in the U.S.

For more information about JEOL USA, Inc. or any JEOL products, visit www.jeolusa.com, or call 978-535-5900.

For more information, please click here

Contacts:
JEOL USA, Inc.
11 Dearborn Road
Peabody, MA
978-535-5900

Patricia Corkum
978-536-2273

Copyright © JEOL

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Researchers find new way to control light with electric fields May 25th, 2017

Nanometrics Announces Retirement Plans of CEO Timothy Stultz: Dr. Stultz to Continue as Director May 25th, 2017

Nanomechanics, Inc. to Exhibit at the SEM Conference: Nanoindentation experts will attend and exhibit their instruments at the Conference and Exposition on Experimental and Applied Mechanics in Indianapolis May 25th, 2017

Three-dimensional graphene: Experiment at BESSY II shows that optical properties are tuneable May 24th, 2017

Announcements

Researchers find new way to control light with electric fields May 25th, 2017

Nanometrics Announces Retirement Plans of CEO Timothy Stultz: Dr. Stultz to Continue as Director May 25th, 2017

Nanomechanics, Inc. to Exhibit at the SEM Conference: Nanoindentation experts will attend and exhibit their instruments at the Conference and Exposition on Experimental and Applied Mechanics in Indianapolis May 25th, 2017

Three-dimensional graphene: Experiment at BESSY II shows that optical properties are tuneable May 24th, 2017

Tools

Nanometrics Announces Retirement Plans of CEO Timothy Stultz: Dr. Stultz to Continue as Director May 25th, 2017

Nanomechanics, Inc. to Exhibit at the SEM Conference: Nanoindentation experts will attend and exhibit their instruments at the Conference and Exposition on Experimental and Applied Mechanics in Indianapolis May 25th, 2017

Plasmon-powered upconversion nanocrystals for enhanced bioimaging and polarized emission: Plasmonic gold nanorods brighten lanthanide-doped upconversion superdots for improved multiphoton bioimaging contrast and enable polarization-selective nonlinear emissions for novel nanoscal May 19th, 2017

The brighter side of twisted polymers: Conjugated polymers designed with a twist produce tiny, brightly fluorescent particles with broad applications May 16th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project