Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Carnegie Mellon Scientists Create Toolbox Of Fluorescent Probes in a Rainbow of Colors

The above figure illustrates three populations of yeast cells labeled with green, orange and red fluoromodules expressed on the cell surface.
The above figure illustrates three populations of yeast cells labeled with green, orange and red fluoromodules expressed on the cell surface.

Abstract:
Scientists at Carnegie Mellon University's Department of Chemistry and Molecular Biosensor and Imaging Center (MBIC) are advancing the state-of-the-art in live cell fluorescent imaging by developing a new class of fluorescent probes that span the spectrum — from violet to the near-infrared.

Carnegie Mellon Scientists Create Toolbox Of Fluorescent Probes in a Rainbow of Colors

San Francisco, CA | Posted on March 26th, 2010

The new technology, called fluoromodules, can be used to monitor biological activities of individual proteins in living cells in real time. At the 239th national meeting of the American Chemical Society, Carnegie Mellon chemists and MBIC scientists will discuss recent advances in their fluoromodule technology that have produced diverse and photostable probes.

Fluoromodules, which consist of dye-protein complexes, provide alternatives to common fluorescent proteins, such as Green Fluorescent Protein (GFP), but with a wider selection of colors and the potential for significantly greater photostability, which allows scientists to image the dye for longer periods of time. This is made possible by the fact that the dye is noncovalently bound to the protein, which allows fresh dye to replace bleached dye.

"We initially isolated and characterized fluoromodules that generate fluorescence from the fluorogenic dyes thiazole orange and malachite green. We are now expanding our repertoire by synthesizing new dyes that emit in the orange and violet regions of the spectrum, and engineering proteins that bind to the new dyes with great affinity," said Chemistry Professor Bruce Armitage, co-director of the Center for Nucleic Acid Science and Technology at Carnegie Mellon and a member of the MBIC team developing the fluoromodules.

Fluoromodules are made of a fluorogen-activating protein (FAP) and a non-fluorescent dye called a fluorogen. The FAP, which is genetically expressed in a cell and tagged to a protein of interest, does not become fluorescent until it binds with its fluorogen. With the novel FAPs and associated fluorogens created by the MBIC team, the researchers can control when a target protein lights up, allowing them to track proteins on the cell surface and within living cells in very simple and direct ways, eliminating cumbersome experimental steps.

Recent advances in the MBIC fluoromodule technology being presented at the ACS meeting include:

> Working with a FAP that had a low affinity for the fluorogenic dye dimethlindole red (DIR), graduate student Hayriye Özhalici-Ünal used PCR mutagenesis to introduce mutations into the FAP's genetic sequence. A small number of mutations increased several-fold the protein's affinity for DIR, enabling very specific and selective binding of the FAP to its dye partner (DIR). Özhalici-Ünal will present this work at 9:50 a.m., Thursday, March 25 during the Follow-on Biologics: Protein Engineering session located in room 201 West Bldg. in the Moscone Center.

> Graduate student Nathaniel Shank synthesized a modified DIR, making it eight-times more photostable. This significant improvement could have an impact on single molecule imaging. Additionally, the modified DIR emits in the orange range of the spectrum, adding another color to the fluoromodule toolkit being developed at MBIC. Shank will present this work at 8 p.m., Tuesday, March 23 during the Total Synthesis of Complex Molecules, Material Devices & Switches, Physical Organic Chemistry poster session located in Hall D of the Moscone Center.

> By synthesizing a new dye and identifying FAPs that bind to it, research chemist Gloria Silva and graduate student Kim Zanotti developed a fluoromodule that emits fluorescence in the violet, which is a welcome addition to a very limited number of probes able to emit in the violet portion of the spectrum. Zanotti will present this work a 6 p.m., Tuesday, March 23 during the poster session located in room 3009/3011 West Bldg. in the Moscone Center.

The aforementioned work, funded by the Pennsylvania Department of Health and the National Institutes of Health (NIH), is part of the mission of the NIH National Technology Center for Networks and Pathways. The effort, headquartered at Carnegie Mellon, is a partnership between Carnegie Mellon and the University of Pittsburgh. For more information, visit www.mbic.cmu.edu/

####

For more information, please click here

Contacts:
Jocelyn Duffy
412-268-9982

Copyright © Carnegie Mellon University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Study finds long-term survival of human neural stem cells transplanted into primate brain April 23rd, 2014

High-Performance, Low-Cost Ultracapacitors Built with Graphene and Carbon Nanotubes: Future devices based on technology could bridge gap between batteries and conventional capacitors in portable electronics and hybrid electric vehicles April 23rd, 2014

Guo Lab Shows Potential of RNA as Heat-resistant Polymer Material for Nanoarchitectures April 23rd, 2014

National Space Society Congratulates SpaceX on the Success of CRS-3 and the First Flight of the Falcon 9R April 22nd, 2014

Govt.-Legislation/Regulation/Funding/Policy

High-Performance, Low-Cost Ultracapacitors Built with Graphene and Carbon Nanotubes: Future devices based on technology could bridge gap between batteries and conventional capacitors in portable electronics and hybrid electric vehicles April 23rd, 2014

Guo Lab Shows Potential of RNA as Heat-resistant Polymer Material for Nanoarchitectures April 23rd, 2014

Cloaked DNA nanodevices survive pilot mission: Successful foray opens door to virus-like DNA nanodevices that could diagnose diseased tissues and manufacture drugs to treat them April 22nd, 2014

Berkeley Lab Researchers Demonstrate First Size-based Chromatography Technique for the Study of Living Cells April 22nd, 2014

Possible Futures

Virus structure inspires novel understanding of onion-like carbon nanoparticles April 10th, 2014

Local girl does good March 22nd, 2014

Surface Characteristics Influence Cellular Growth on Semiconductor Material March 12th, 2014

The "Tipping Point" February 12th, 2014

Academic/Education

Global leader in solar cell manufacturing eyes New York for major expansion outside of Japan: CNSE and Solar Frontier Explore $700 Million Investment, Job Creation in New York State April 22nd, 2014

University of Waterloo Visits China to Strengthen Bonds With Research Partners April 21st, 2014

Director Wally Pfister joins UC Berkeley neuroengineers to discuss the science behind ‘Transcendence’ April 7th, 2014

First annual science week highlights STEM pipeline and partnerships: UB, SUNY Buffalo State and ECC team up with the City of Buffalo and its schools for April 7-11 events April 3rd, 2014

Nanomedicine

Study finds long-term survival of human neural stem cells transplanted into primate brain April 23rd, 2014

Cloaked DNA nanodevices survive pilot mission: Successful foray opens door to virus-like DNA nanodevices that could diagnose diseased tissues and manufacture drugs to treat them April 22nd, 2014

Berkeley Lab Researchers Demonstrate First Size-based Chromatography Technique for the Study of Living Cells April 22nd, 2014

Amino-functionalized carbon nanotubes act as a carrier for nerve growth factor April 21st, 2014

Sensors

Iranian Researchers Present New Model to Strengthen Superconductivity at Higher Temperatures April 19th, 2014

Transparent Conductive Films and Sensors Are Hot Segments in Printed Electronics: Start-ups in these fields show above-average momentum, while companies working on emissive displays such as OLED are fading, Lux Research says April 17th, 2014

Biologists Develop Nanosensors to Visualize Movements and Distribution of Plant Stress Hormone April 15th, 2014

LetiDays Grenoble to Present Multiple Perspectives on Development, Challenges and Markets for the IoT April 14th, 2014

Announcements

Study finds long-term survival of human neural stem cells transplanted into primate brain April 23rd, 2014

High-Performance, Low-Cost Ultracapacitors Built with Graphene and Carbon Nanotubes: Future devices based on technology could bridge gap between batteries and conventional capacitors in portable electronics and hybrid electric vehicles April 23rd, 2014

Guo Lab Shows Potential of RNA as Heat-resistant Polymer Material for Nanoarchitectures April 23rd, 2014

National Space Society Congratulates SpaceX on the Success of CRS-3 and the First Flight of the Falcon 9R April 22nd, 2014

Nanobiotechnology

Guo Lab Shows Potential of RNA as Heat-resistant Polymer Material for Nanoarchitectures April 23rd, 2014

Cloaked DNA nanodevices survive pilot mission: Successful foray opens door to virus-like DNA nanodevices that could diagnose diseased tissues and manufacture drugs to treat them April 22nd, 2014

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Biologists Develop Nanosensors to Visualize Movements and Distribution of Plant Stress Hormone April 15th, 2014

Research partnerships

University of Waterloo Visits China to Strengthen Bonds With Research Partners April 21st, 2014

Novel stapled peptide nanoparticle combination prevents RSV infection, study finds April 17th, 2014

Scientists Capture Ultrafast Snapshots of Light-Driven Superconductivity: X-rays reveal how rapidly vanishing 'charge stripes' may be behind laser-induced high-temperature superconductivity April 16th, 2014

Scalable CVD process for making 2-D molybdenum diselenide: Rice, NTU scientists unveil CVD production for coveted 2-D semiconductor April 8th, 2014

NanoNews-Digest
The latest news from around the world, FREE







  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE