Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Carnegie Mellon Scientists Create Toolbox Of Fluorescent Probes in a Rainbow of Colors

The above figure illustrates three populations of yeast cells labeled with green, orange and red fluoromodules expressed on the cell surface.
The above figure illustrates three populations of yeast cells labeled with green, orange and red fluoromodules expressed on the cell surface.

Abstract:
Scientists at Carnegie Mellon University's Department of Chemistry and Molecular Biosensor and Imaging Center (MBIC) are advancing the state-of-the-art in live cell fluorescent imaging by developing a new class of fluorescent probes that span the spectrum — from violet to the near-infrared.

Carnegie Mellon Scientists Create Toolbox Of Fluorescent Probes in a Rainbow of Colors

San Francisco, CA | Posted on March 26th, 2010

The new technology, called fluoromodules, can be used to monitor biological activities of individual proteins in living cells in real time. At the 239th national meeting of the American Chemical Society, Carnegie Mellon chemists and MBIC scientists will discuss recent advances in their fluoromodule technology that have produced diverse and photostable probes.

Fluoromodules, which consist of dye-protein complexes, provide alternatives to common fluorescent proteins, such as Green Fluorescent Protein (GFP), but with a wider selection of colors and the potential for significantly greater photostability, which allows scientists to image the dye for longer periods of time. This is made possible by the fact that the dye is noncovalently bound to the protein, which allows fresh dye to replace bleached dye.

"We initially isolated and characterized fluoromodules that generate fluorescence from the fluorogenic dyes thiazole orange and malachite green. We are now expanding our repertoire by synthesizing new dyes that emit in the orange and violet regions of the spectrum, and engineering proteins that bind to the new dyes with great affinity," said Chemistry Professor Bruce Armitage, co-director of the Center for Nucleic Acid Science and Technology at Carnegie Mellon and a member of the MBIC team developing the fluoromodules.

Fluoromodules are made of a fluorogen-activating protein (FAP) and a non-fluorescent dye called a fluorogen. The FAP, which is genetically expressed in a cell and tagged to a protein of interest, does not become fluorescent until it binds with its fluorogen. With the novel FAPs and associated fluorogens created by the MBIC team, the researchers can control when a target protein lights up, allowing them to track proteins on the cell surface and within living cells in very simple and direct ways, eliminating cumbersome experimental steps.

Recent advances in the MBIC fluoromodule technology being presented at the ACS meeting include:

> Working with a FAP that had a low affinity for the fluorogenic dye dimethlindole red (DIR), graduate student Hayriye Özhalici-Ünal used PCR mutagenesis to introduce mutations into the FAP's genetic sequence. A small number of mutations increased several-fold the protein's affinity for DIR, enabling very specific and selective binding of the FAP to its dye partner (DIR). Özhalici-Ünal will present this work at 9:50 a.m., Thursday, March 25 during the Follow-on Biologics: Protein Engineering session located in room 201 West Bldg. in the Moscone Center.

> Graduate student Nathaniel Shank synthesized a modified DIR, making it eight-times more photostable. This significant improvement could have an impact on single molecule imaging. Additionally, the modified DIR emits in the orange range of the spectrum, adding another color to the fluoromodule toolkit being developed at MBIC. Shank will present this work at 8 p.m., Tuesday, March 23 during the Total Synthesis of Complex Molecules, Material Devices & Switches, Physical Organic Chemistry poster session located in Hall D of the Moscone Center.

> By synthesizing a new dye and identifying FAPs that bind to it, research chemist Gloria Silva and graduate student Kim Zanotti developed a fluoromodule that emits fluorescence in the violet, which is a welcome addition to a very limited number of probes able to emit in the violet portion of the spectrum. Zanotti will present this work a 6 p.m., Tuesday, March 23 during the poster session located in room 3009/3011 West Bldg. in the Moscone Center.

The aforementioned work, funded by the Pennsylvania Department of Health and the National Institutes of Health (NIH), is part of the mission of the NIH National Technology Center for Networks and Pathways. The effort, headquartered at Carnegie Mellon, is a partnership between Carnegie Mellon and the University of Pittsburgh. For more information, visit www.mbic.cmu.edu/

####

For more information, please click here

Contacts:
Jocelyn Duffy
412-268-9982

Copyright © Carnegie Mellon University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Effective Nanotechnology Innovations to Receive Mustafa Prize September 16th, 2014

‘Small’ transformation yields big changes September 16th, 2014

Elusive Quantum Transformations Found Near Absolute Zero: Brookhaven Lab and Stony Brook University researchers measured the quantum fluctuations behind a novel magnetic material's ultra-cold ferromagnetic phase transition September 15th, 2014

'Squid skin' metamaterials project yields vivid color display: Rice lab creates RGB color display technology with aluminum nanorods September 15th, 2014

Govt.-Legislation/Regulation/Funding/Policy

‘Small’ transformation yields big changes September 16th, 2014

'Squid skin' metamaterials project yields vivid color display: Rice lab creates RGB color display technology with aluminum nanorods September 15th, 2014

Fonon at Cutting-Edge of 3D Military Printing: Live-Combat Scenarios Could See a Decisive Advantage with 3D Printing September 15th, 2014

Seeking Nanoscale Defenses for Biological and Chemical Threats: WPI co-organizes a NATO workshop to improve the detection and decontamination of biological and chemical agents September 13th, 2014

Possible Futures

Air Force’s 30-year plan seeks 'strategic agility' August 1st, 2014

IBM Announces $3 Billion Research Initiative to Tackle Chip Grand Challenges for Cloud and Big Data Systems: Scientists and engineers to push limits of silicon technology to 7 nanometers and below and create post-silicon future July 10th, 2014

Virus structure inspires novel understanding of onion-like carbon nanoparticles April 10th, 2014

Local girl does good March 22nd, 2014

Academic/Education

Malvern technology delivers Malvern reliability in multi-disciplinary lab at Queen Mary University London September 9th, 2014

State University of New York Trustees Unanimously Approve SUNY Polytechnic Institute (SUNY Poly) as New Name for Merged SUNY CNSE / SUNYIT September 9th, 2014

New Vice President Takes Helm at CNSE CMOST: Catherine Gilbert To Lead CNSE Children’s Museum of Science and Technology Through Expansion And Relocation August 29th, 2014

RMIT delivers $30m boost to micro and nano-tech August 26th, 2014

Nanomedicine

Treatment of Cell Infection by Nanotechnology September 15th, 2014

Researchers Create World’s Largest DNA Origami September 11th, 2014

Iranian Scientists Discover Nanotechnology Method to Remove Limitations in Tumor Surgery September 11th, 2014

Iranian Nanotechnology Scientists Produce Polymeric Scaffolds for Tissue Engineering September 11th, 2014

Sensors

Simple, Cost-Effective Method Proposed for Synthesizing Zinc Oxide Nanopigments September 15th, 2014

First Colloid and Polymer Science Lecture awarded to Orlin D. Velev: Chemical engineer honored for outstanding research in colloid science September 12th, 2014

UT Arlington research uses nanotechnology to help cool electrons with no external sources September 11th, 2014

Development of Algorithm for Accurate Calculation of Average Distance Travelled by Low-Speed Electrons without Energy Loss that Are Sensitive to Surface Structure September 11th, 2014

Announcements

Effective Nanotechnology Innovations to Receive Mustafa Prize September 16th, 2014

‘Small’ transformation yields big changes September 16th, 2014

Rice rolls 'neat' nanotube fibers: Rice University researchers' acid-free approach leads to strong conductive carbon threads September 15th, 2014

Simple, Cost-Effective Method Proposed for Synthesizing Zinc Oxide Nanopigments September 15th, 2014

Nanobiotechnology

NanoStruck has a High Recovery Rate on Mine Tailings: retrieval of up to 96% of Gold, 88% of Silver and 86% of Palladium September 12th, 2014

Boosting armor for nuclear-waste eating microbes September 12th, 2014

Researchers Create World’s Largest DNA Origami September 11th, 2014

UO-Berkeley Lab unveil new nano-sized synthetic scaffolding technique: Oil-and-water approach from Richmond's UO lab to spark new line of versatile peptoid nanosheets September 2nd, 2014

Research partnerships

Elusive Quantum Transformations Found Near Absolute Zero: Brookhaven Lab and Stony Brook University researchers measured the quantum fluctuations behind a novel magnetic material's ultra-cold ferromagnetic phase transition September 15th, 2014

NEI Corporation and PneumatiCoat Technologies Sign Agreement to Jointly Develop and Market New Materials for Lithium-ion Batteries September 12th, 2014

Advanced Light Source Sets Microscopy Record| Berkeley Lab Researchers Achieve Highest Resolution Ever with X-ray Microscopy September 11th, 2014

Researchers Create World’s Largest DNA Origami September 11th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE