Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Paper of Special Interest: Nanofluidics Identify Epigenetic Changes One Molecule at a Time

Abstract:
Using a system of nanofluidic channels and multicolor fluorescence microscopy, a team of investigators at Cornell University has developed a method that analyzes the binding of DNA and DNA-binding proteins known as histones at specific locations along individual DNA molecules. The data generated using this method provides information on the so-called epigenetic state of a cell, which reflect differences in the genes that a given cell is expressing at any one time.

Paper of Special Interest: Nanofluidics Identify Epigenetic Changes One Molecule at a Time

Bethesda, MD | Posted on March 22nd, 2010

This research effort was led by Paul Soloway, Ph.D., and Harold Craighead, Ph.D., who is also the principle investigator of the Cornell University Physical Sciences-Oncology Center, one of eight newly established centers funded by the National Cancer Institute to identify and study the physical and biological laws and principles that guide the development and spread of cancer. The investigators published the results of this project in the journal Analytical Chemistry.

Every cell in the body contains the same genetic blueprint, but what differentiates a liver cell from a heart cell is a series of DNA modifications, such as methylation, that determines the specific set of genes that are expressed in a specific type of cell. These modifications are known as epigenetic, rather than genetic, changes since they don't alter DNA's sequence, just its structural properties. Those structural changes determine which genes are accessible to the many proteins involved in turning genetic information into specific proteins.

There are many techniques that researchers can use to probe such epigenetic changes, but these methods require large numbers of cells, and thus, produce an average picture of epigenetic state. In addition, these techniques cannot survey the entire genome, nor can they examine two different types of epigenetic changes simultaneously.

To solve these limitations, the Cornell team created a nanofluidic device capable of flowing individual DNA molecules through a channel and past a detector that can record and analyze the fluorescence of DNA and its associated proteins in real time. The researchers also demonstrated that they can take DNA stripped of its proteins, label it with a fluorescent molecule that binds to methylated bases, and detect specific locations of DNA methylation.

In this set of experiments, the researchers used their nanofluidic system to reveal the frequency and coincidence of epigenetic changes in single DNA molecules. The investigators believe, however, that they will be able to modify the device to rapidly sort DNA-protein structures based on their epigenetic signatures. The sorted chromatin fragments could then be studied further using all the tools of DNA, including DNA sequencing.

his work is detailed in a paper titled, "Single Molecule Epigenetic Analysis in a Nanofluidic Channel." An abstract of this paper is available at the journal's Web site.

View abstract: pubs.acs.org/doi/abs/10.1021/ac9028642

####

About NCI Alliance for Nanotechnology in Cancer
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Copyright © NCI Alliance for Nanotechnology in Cancer

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Promising Step Taken in Iran towards Treatment of Spinal Cord Injury August 3rd, 2015

Diagnosis of Salmonella Bacterium-Caused Food Poisoning by Biosensors August 3rd, 2015

Thin films offer promise for ferroelectric devices: Researchers at Tokyo Institute of Technology demystify the ferroelectric properties observed in hafnium-oxide-based thin films, revealing a potentially useful device material August 3rd, 2015

Kalam: versatility personified August 1st, 2015

Possible Futures

Nanofiltration Membrane Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Nanozirconia Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Self-Healing Nano Anti-rust Coatings Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Nano Spray Instrument Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Academic/Education

Pakistani Students Who Survived Terror Attack to Attend Weeklong “NanoDiscovery Institute” at SUNY Poly CNSE in Albany July 29th, 2015

Deben reports on the use of their CT500 in the X-ray microtomography laboratory at La Trobe University, Melbourne, Australia July 22nd, 2015

JPK reports on the use of SPM in the Messersmith Group at UC Berkeley looking at biologically inspired polymer adhesives. July 21st, 2015

Renishaw adds Raman analysis to Scanning Electron Microscopy at the University of Sydney, Australia July 9th, 2015

Nanomedicine

Promising Step Taken in Iran towards Treatment of Spinal Cord Injury August 3rd, 2015

Diagnosis of Salmonella Bacterium-Caused Food Poisoning by Biosensors August 3rd, 2015

Gold-diamond nanodevice for hyperlocalised cancer therapy: Gold nanorods can be used as remote controlled nanoheaters delivering the right amount of thermal treatment to cancer cells, thanks to diamond nanocrystals used as temperature sensors August 1st, 2015

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

Announcements

Promising Step Taken in Iran towards Treatment of Spinal Cord Injury August 3rd, 2015

Diagnosis of Salmonella Bacterium-Caused Food Poisoning by Biosensors August 3rd, 2015

Thin films offer promise for ferroelectric devices: Researchers at Tokyo Institute of Technology demystify the ferroelectric properties observed in hafnium-oxide-based thin films, revealing a potentially useful device material August 3rd, 2015

Advances and Applications in Biosensing, Sensor Power, and Sensor R&D to be Covered at Sensors Global Summit August 1st, 2015

Nanobiotechnology

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

European Technology Platform for Nanomedicine and ENATRANS European Consortium Launch the 2nd edition of the Nanomedicine Award: The Award to be presented at BIO-Europe conference in Munich, November 2015 July 30th, 2015

New computer model could explain how simple molecules took first step toward life: Two Brookhaven researchers developed theoretical model to explain the origins of self-replicating molecules July 28th, 2015

Spintronics: Molecules stabilizing magnetism: Organic molecules fixing the magnetic orientation of a cobalt surface/ building block for a compact and low-cost storage technology/ publication in Nature Materials July 25th, 2015

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project