Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > 3-D cell culture: making cells feel right at home

Abstract:
Startup Nano3D Bio's system based on Rice-M.D. Anderson tech

Research in this week's Nature Nanotechnology takes aim at a biological icon: the two-dimensional petri dish. Scientists from Rice University and the University of Texas M.D. Anderson Cancer Center have found a simple way to suspend cells in magnetic fields so they grow into three-dimensional cell cultures. Compared with flat cell cultures, the 3-D cell cultures more closely resemble real tissues from the body and should provide more realistic targets for testing new drugs.

3-D cell culture: making cells feel right at home

Houston, TX | Posted on March 15th, 2010

The film "Avatar" isn't the only 3-D blockbuster making a splash this winter. A team of scientists from Houston's Texas Medical Center this week unveiled a new technique for growing 3-D cell cultures, a technological leap from the flat petri dish that could save millions of dollars in drug-testing costs. The research is reported in Nature Nanotechnology.

The 3-D technique is easy enough for most labs to set up immediately. It uses magnetic forces to levitate cells while they divide and grow. Compared with cell cultures grown on flat surfaces, the 3-D cell cultures tend to form tissues that more closely resemble those inside the body.

"There's a big push right now to find ways to grow cells in 3-D because the body is 3-D, and cultures that more closely resemble native tissue are expected to provide better results for preclinical drug tests," said study co-author Tom Killian, associate professor of physics at Rice. "If you could improve the accuracy of early drug screenings by just 10 percent, it's estimated you could save as much as $100 million per
drug."

For cancer research, the "invisible scaffold" created by the magnetic field goes beyond its potential for producing cell cultures that are more reminiscent of real tumors, which itself would be an important advance, said co-author Wadih Arap, professor in the David H. Koch Center at The University of Texas M.D. Anderson Cancer Center.

To make cells levitate, the research team modified a combination of gold nanoparticles and engineered viral particles called "phage" that was developed in the lab of Arap and Renata Pasqualini, also of the Koch Center. This targeted "nanoshuttle" can deliver payloads to specific organs or tissues.

"A logical next step for us will be to use this additional magnetic property in targeted ways to explore possible applications in the imaging and treatment of tumors," Arap said.

The 3-D modeling raises another interesting long-term possibility. "This is a step toward building better models of organs in the lab," Pasqualini said.

The new technique is an example of the innovation that can result when experts come together from disparate fields. Killian studies ultracold atoms and uses finely tuned magnetic fields to manipulate them. He had been working with Rice bioengineer Robert Raphael for several years on methods to use magnetic fields to manipulate cells. So when Killian's friend Glauco Souza, then an Odyssey Scholar studying with Arap and Pasqualini, mentioned one day that he was developing a gel that could load cancer cells with magnetic nanoparticles, it led to a new idea.

"We wondered if we might be able to use magnetic fields to manipulate the cells after my gels put magnetic nanoparticles into them," said Souza, who left M.D. Anderson in 2009 to co-found Nano3D Biosciences (www.n3dbio.com), a startup that subsequently licensed the technology from Rice and M.D. Anderson.

The nanoparticles in this case are tiny bits of iron oxide. These are added to a gel that contains phage. When cells are added to the gel, the phage causes the particles to be absorbed into cells over a few hours. The gel is then washed away, and the nanoparticle-loaded cells are placed in a petri dish filled with a liquid that promotes cell growth and division.

In the new study, the researchers showed that by placing a coin-sized magnet atop the dish's lid, they could lift the cells off the bottom of the dish, concentrate them and allow them to grow and divide while they were suspended in the liquid.

A key experiment was performed in collaboration with Jennifer Molina, a graduate student in the laboratory of Maria-Magdalena Georgescu, an M.D. Anderson associate professor in neuro-oncology and also a co-author, in which the technique was used on brain tumor cells called glioblastomas. The results showed that cells grown in the 3-D medium produced proteins that were similar to those produced by gliobastoma tumors in mice, while cells grown in 2-D did not show this similarity.

Souza said that Nano3D Biosciences is conducting additional tests to compare how the new method stacks up against existing methods of growing 3-D cell cultures. He said he is hopeful that it will provide results that are just as good, if not better, than longstanding techniques that use 3-D scaffolds.

Raphael, a paper co-author, associate professor in bioengineering and a member of Rice's BioScience Research Collaborative, said, "The beauty of this method is that it allows natural cell-cell interactions to drive assembly of 3-D microtissue structures. The method is fairly simple and should be a good point of entry in 3-D cell culturing for any lab that's interested in drug discovery, stem cell biology, regenerative medicine or biotechnology."

Other co-authors include Daniel Stark and Jeyarama Ananta, both of Rice; Carly Levin of Nano3D Biosciences; and Michael Ozawa, Lawrence Bronk, Jami Mandelin, James Bankson and Juri Gelovani, all of M.D. Anderson.

The research was funded by M.D. Anderson's Odyssey Scholar Program, the Department of Defense's Breast Cancer Research Program, the National Science Foundation, the Packard Foundation, the Gillson-Longenbaugh Foundation, AngelWorks, the National Institutes of Health and the National Cancer Institute.

####

About Rice University
Located in Houston, Rice University is consistently ranked one of America's best teaching and research universities. Known for its "unconventional wisdom," Rice is distinguished by its: size -- 3,102 undergraduates and 2,237 graduate students; selectivity -- 12 applicants for each place in the freshman class; resources -- an undergraduate student-to-faculty ratio of 5-to-1; sixth largest endowment per student among American private research universities; residential college system, which builds communities that are both close-knit and diverse; and collaborative culture, which crosses disciplines, integrates teaching and research, and intermingles undergraduate and graduate work. To learn more, visit www.rice.edu.

Who Knew? explore.rice.edu/explore/Who_Knew.asp

About M. D. Anderson
The University of Texas M. D. Anderson Cancer Center in Houston ranks as one of the world's most respected centers focused on cancer patient care, research, education and prevention. M. D. Anderson is one of only 39 Comprehensive Cancer Centers designated by the National Cancer Institute. For six of the past eight years, M. D. Anderson has ranked No. 1 in cancer care in “America's Best Hospitals,” a survey published annually in U.S. News and World Report. To learn more, visit www.mdanderson.org.

For more information, please click here

Contacts:
Jade Boyd
713-348-6778


Scott Merville
713-792-0661

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

The next step in DNA computing: GPS mapping? May 6th, 2015

Improving Clinical Care and Patient Quality of Life in Advanced Liver Disease, d-LIVER Workshop, Milan, 27 May 2015 May 6th, 2015

Grafoid Acquires MuAnalysis Inc; Expands Its Advanced Materials Testing Capabilities May 6th, 2015

Winner Announced for NNI’s First ‘EnvisioNano’ Nanotechnology Image Contest May 6th, 2015

Govt.-Legislation/Regulation/Funding/Policy

Channeling valleytronics in graphene: Berkeley Lab researchers discover 1-D conducting channels in bilayer graphene May 6th, 2015

A better way to build DNA scaffolds: McGill researchers devise new technique to produce long, custom-designed DNA strands May 6th, 2015

Thermometer-like device could help diagnose heart attacks May 6th, 2015

Winner Announced for NNI’s First ‘EnvisioNano’ Nanotechnology Image Contest May 6th, 2015

Possible Futures

Printing Silicon on Paper, with Lasers April 21st, 2015

A glass fiber that brings light to a standstill: By coupling photons to atoms, light in a glass fiber can be slowed down to the speed of an express train; for a short while it can even be brought to a complete stop April 9th, 2015

Nanotechnology in Medical Devices Market is expected to reach $8.5 Billion by 2019 March 25th, 2015

Nanotechnology Enabled Drug Delivery to Influence Future Diagnosis and Treatments of Diseases March 21st, 2015

Academic/Education

New JEOL E-Beam Lithography System to Enhance Quantum NanoFab Capabilities May 6th, 2015

FEI Partners With the George Washington University to Equip New Science & Engineering Hall: Suite of new high-performance microscopes will be used for cutting-edge experiments at GW’s new research facility April 29th, 2015

Renishaw Raman systems used to study 2D materials at Boston University, Massachusetts, USA. April 28th, 2015

SUNY Poly and Sematech Announce Air Products Joins Cutting-Edge CMP Center At Albany Nanotech Complex April 28th, 2015

Nanomedicine

A better way to build DNA scaffolds: McGill researchers devise new technique to produce long, custom-designed DNA strands May 6th, 2015

Thermometer-like device could help diagnose heart attacks May 6th, 2015

Improving Clinical Care and Patient Quality of Life in Advanced Liver Disease, d-LIVER Workshop, Milan, 27 May 2015 May 6th, 2015

Arrowhead Completes Dosing Healthy Volunteers and Initiates Transition to Patients in Phase 1 Study of ARC-AAT May 5th, 2015

Announcements

The next step in DNA computing: GPS mapping? May 6th, 2015

Improving Clinical Care and Patient Quality of Life in Advanced Liver Disease, d-LIVER Workshop, Milan, 27 May 2015 May 6th, 2015

Grafoid Acquires MuAnalysis Inc; Expands Its Advanced Materials Testing Capabilities May 6th, 2015

Winner Announced for NNI’s First ‘EnvisioNano’ Nanotechnology Image Contest May 6th, 2015

Nanobiotechnology

A better way to build DNA scaffolds: McGill researchers devise new technique to produce long, custom-designed DNA strands May 6th, 2015

The next step in DNA computing: GPS mapping? May 6th, 2015

Improving Clinical Care and Patient Quality of Life in Advanced Liver Disease, d-LIVER Workshop, Milan, 27 May 2015 May 6th, 2015

Artificial photosynthesis could help make fuels, plastics and medicine April 29th, 2015

Research partnerships

New chip architecture may provide foundation for quantum computer: Researchers at the Georgia Tech Research Institute have developed a microfabricated ion trap architecture that holds promise for increasing the density of qubits in future quantum computers May 5th, 2015

'Microcombing' creates stronger, more conductive carbon nanotube films May 5th, 2015

Silicon Storage Technology and GLOBALFOUNDRIES Announce Qualification of Automotive Grade 55nm Embedded Flash Memory Technology May 5th, 2015

Electron chirp: Cyclotron radiation from single electrons measured directly for first time: Method has potential to measure neutrino mass and look beyond the Standard Model of the universe April 29th, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project