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Abstract:
University of Calgary chemist finds right mix of tools to measure nanomaterials in blood vessels

Vigilance needed in nanotechnology

Calgary | Posted on March 4th, 2010

University of Calgary chemistry professor David Cramb is a step closer to helping solve a complex problem in nanotechnology: the impact nanoparticles have on human health and the environment.

Cramb, director of the Faculty of Science's nanoscience program, and his researchers have developed a methodology to measure various aspects of nanoparticles in the blood stream of chicken embryos. Their discovery is published in the March online edition of Chemical Physics Letters. www.sciencedirect.com/science/journal/00092614.

"With the boom in nanomaterials production there is an increasing possibility of environmental and/or human exposure. Thus there is a need to investigate their potential detrimental effects," says Cramb. "We have developed very specialized tools to begin measuring such impacts."

Nanoparticles are particles or groups of atoms or molecules nanometers in size. One millimetre (or the diameter of the head of a pin) is equal to one-million nanometres. Nanoparticles are already used in the cosmetics industry and are being developed for drug delivery, diagnostic imaging and tissue engineering, to name only a few applications. It is estimated investments in nanotechnology globally will reach about $12 trillion US by 2012.

Cramb is looking for ways to help answer questions including: If embryos are exposed to nanoparticles, where will the nanoparticles go? How will the embryo respond? What regulatory approaches can be recommended to mitigate accidental exposure? How can nanotechnology be made green and sustainable?

"Bioaccumulation studies involving embryos are being conducted in our laboratory," says Cramb. "These studies are important since chronic nanotoxicity in an adult organism could be related to exposure during the development process. Additionally, acute exposure may affect embryonic viability."

Cramb and his researchers studied motion and light induced changes in nanoparticles by focusing a laser beam into a blood vessel containing nanoparticles and measuring fluorescence. (The measurements provide a determination of particles aggregation in the vessel). This is unique because it has never been done in a live embryo. The results will now allow measurement and understanding of uptake into embryonic tissues.

The Organization for Economic Co-operation and Development is leading a global effort to develop research that will help member states to implement sound, science-based regulatory frameworks for the burgeoning nanotechnology industry. NSERC and Canadian Institutes of Health Research (CHRP - Collaborative Health Research Projects Program) have provided $600,000 disbursed over three years to fund research in this area by a team including the research groups of Cramb, Kristina Rinker and Sarah Childs of the University of Calgary and Warren Chan of the University of Toronto.

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About University of Calgary
The University of Calgary is a comprehensive research university, ranked one of Canada's top seven research universities, combining the best of long-established university traditions with the city of Calgary's vibrant energy and diversity.

The U of C campus occupies a beautiful, park-like setting covering more than 200 hectares, an area larger than Calgary's entire downtown. The university is home to scholars in 17 faculties (offering more than 100 academic programs) and more than 30 research institutes and centres.

More than 27,600 students are currently enrolled in undergraduate, graduate and professional degree programs. The U of C has graduated 135,000 alumni over its 43-year history, including the Prime Minister of Canada, Stephen Harper.

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