Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Incorrectly Folded Fibers

Abstract:
Flash frozen under the electron microscope: examining the mechanical properties of Alzheimer's amyloid fibrils

Incorrectly Folded Fibers

Weinheim, Germany | Posted on January 26th, 2010

Alzheimer's disease, Parkinson's disease, type-II diabetes, and prion diseases like BSE all involve the deposition of amyloid fibrils in tissues and organs. These are fibrous clumps of incorrectly folded proteins; their exact structures and their roles in pathological processes are not yet completely understood. By using electron microscopic images of flash frozen samples, researchers have now been able to examine the exact structure of Alzheimer's amyloid fibrils and to assess their mechanical properties. As the team reports in the journal Angewandte Chemie, the fibrils are very stiff—one of the underlying causes of their pathogenicity.

Because amyloid fibrils are very difficult to analyze with traditional biophysical techniques, Marcus Fändrich (Max Planck Unit for Enzymology of Protein Folding, Halle/Saale, Germany), Carsten Sachse (MRC Laboratory of Molecular Biology, Cambridge, UK), and Nikolaus Grigorieff (Brandeis University, Waltham, USA) were forced to take another approach: They examined Alzheimer's amyloid fibrils by electron cryomicroscopy. "These experiments allowed us to examine the structure of the fibrils at a previously unattainable resolution," explains Fändrich.

The fibrils appear in twisted bands about 20 nm wide and are often bent in the raw electron microscopic images. "These bent fibrils are a snapshot of the fibrils in solution," says Fändrich. "We use the degree of bending and twisting to calculate how stiff the fibrils are." This revealed that the Alzheimer's amyloid fibrils are relatively rigid structures. "The uncontrolled formation of such stiff fibrils is presumably critical for the pathogenicity of amyloid fibrils," reports Fändrich. "In many amyloid diseases, the fibrils are preferentially deposited in tissues that are normally contractile or elastic, like the heart muscle or the walls of blood vessels. Medical findings indicate that the fibrils somewhat stiffen these tissues."

"In addition, our data may help to better evaluate the possible uses of amyloid fibers as novel biotechnological agents," reports Fändrich. Based on their material properties and ease of modification, amyloid fibers are potentially interesting as novel building materials.

Author: Marcus Fändrich, Max Planck Research Unit for Enzymology of Protein Folding, Halle (Germany), www.enzyme-halle.mpg.de/amyloid/staff.htm

Title: Nanoscale Flexibility Parameters of Alzheimer Amyloid Fibrils Determined by Electron Cryo-Microscopy

Angewandte Chemie International Edition 2010, 49, No. 7, Permalink: dx.doi.org/10.1002/anie.200904781

####

About Wiley InterScience
Wiley InterScience (www.interscience.wiley.com) provides access to over 3 million articles across nearly 1500 journals and 7000 Online Books and major reference works. It also holds industry leading databases such as The Cochrane Library, chemistry databases and the acclaimed Current Protocols laboratory manuals.

Wiley InterScience is one of the world's premiere resources for study, teaching and advanced research.

For more information, please click here

Contacts:
Editorial office


Amy Molnar (US)


Jennifer Beal (UK)


Alina Boey (Asia)

Copyright © Wiley InterScience

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Organometallics welcomes new editor-in-chief: Paul Chirik, Ph.D. July 22nd, 2014

The Hiden EQP Plasma Diagnostic with on-board MCA July 22nd, 2014

Iran to Hold 3rd Int'l Forum on Nanotechnology Economy July 22nd, 2014

Nanometrics Announces Upcoming Investor Events July 22nd, 2014

Nanomedicine

Researchers create vaccine for dust-mite allergies Main Page Content: Vaccine reduced lung inflammation to allergens in lab and animal tests July 22nd, 2014

NIST shows ultrasonically propelled nanorods spin dizzyingly fast July 22nd, 2014

SentiMag® Now Available in Australia and New Zealand July 21st, 2014

More than glitter: Scientists explain how gold nanoparticles easily penetrate cells, making them useful for delivering drugs July 21st, 2014

Announcements

Nanometrics Announces Upcoming Investor Events July 22nd, 2014

Bruker Awarded Fourth PeakForce Tapping Patent: AFM Mode Uniquely Combines Highest Resolution Imaging and Material Property Mapping July 22nd, 2014

NIST shows ultrasonically propelled nanorods spin dizzyingly fast July 22nd, 2014

Penn Study: Understanding Graphene’s Electrical Properties on an Atomic Level July 22nd, 2014

Nanobiotechnology

Production of Non-Virus Nanocarriers with Highest Amount of Gene Delivery July 17th, 2014

Physicists Use Computer Models to Reveal Quantum Effects in Biological Oxygen Transport: The team solved a long-standing question by explaining why oxygen – and not deadly carbon monoxide – preferably binds to the proteins that transport it around the body. July 17th, 2014

Tiny DNA pyramids enter bacteria easily -- and deliver a deadly payload July 9th, 2014

Artificial cilia: Scientists from Kiel University develop nano-structured transportation system July 4th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE