Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Monitoring Cancer Cell Changes With Quantum Dots

Abstract:
One of the earliest events that changes a normal cell into a malignant one is known as deoxyribonucleic acid (DNA) hypermethylation, a biochemical alteration that inactivates critical tumor-suppressor genes. A team of investigators at Johns Hopkins University has developed a quantum dot-based method that can quantify DNA methylation in premalignant cells harvested from human patients.

Monitoring Cancer Cell Changes With Quantum Dots

Bethesda, MD | Posted on July 21st, 2009

Jeff Tza-Huei Wang, Ph.D., and Hetty E. Carraway, M.D., led the team of researchers that developed the method they call methylation-specific quantitative fluorescence resonance energy transfer (MS-qFRET). The details of their work appear in the journal Genome Research. The MS-qFRET process starts by treating sample DNA with sodium bisulfite, which converts all unmethylated cytosines (one of the four nucleic acid components of DNA) into uracil, leaving any methylated cytosines unchanged. The treated DNA then is amplified using a modified polymerase chain reaction procedure that differentiates between methylated and unmethylated DNA. This procedure also introduces fluorescent markers and biotin molecules on each piece of methylated DNA. Finally, streptavidin-coated quantum dots are added to the amplified DNA, binding tightly to the biotin-linked DNA molecules.

Quantification of methylated DNA occurs by the FRET process, in which energy transfers between the fluorescent molecule and the nearby quantum dot. The amount of fluorescence quenching, measured using confocal microscopy, provides a sensitive and accurate measure of DNA methylation. The technique is sensitive enough to enable the investigators to monitor methylation changes after premalignant cells are treated with drugs known to alter methylation patterns. The researchers also note that this technique is amenable to multiplexing, which affords the opportunity to compare multiple samples from the same patient.

This work, which was supported in part by the National Cancer Institute, is detailed in the paper "MS-qFRET: A quantum dot-based method for analysis of DNA methylation." An investigator from the Lovelace Respiratory Research Institute in Albuquerque also participated in this study. An abstract of the paper is available at the journal's Web site.

####

About NCI Alliance for Nanotechnology in Cancer
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Copyright © NCI Alliance for Nanotechnology in Cancer

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Conductive Inks: booming to $2.8 billion by 2024 April 17th, 2014

High-temperature plasmonics eyed for solar, computer innovation April 17th, 2014

INSCX™ exchange to present Exchange trade reporting mechanism for engineered nanomaterials (NMs) to UK regulation agencies, insurers and upstream/downstream users April 17th, 2014

Transparent Conductive Films and Sensors Are Hot Segments in Printed Electronics: Start-ups in these fields show above-average momentum, while companies working on emissive displays such as OLED are fading, Lux Research says April 17th, 2014

Possible Futures

Virus structure inspires novel understanding of onion-like carbon nanoparticles April 10th, 2014

Local girl does good March 22nd, 2014

Surface Characteristics Influence Cellular Growth on Semiconductor Material March 12th, 2014

The "Tipping Point" February 12th, 2014

Nanomedicine

Novel stapled peptide nanoparticle combination prevents RSV infection, study finds April 17th, 2014

More effective kidney stone treatment, from the macroscopic to the nanoscale April 17th, 2014

High-temperature plasmonics eyed for solar, computer innovation April 17th, 2014

Harris & Harris Group Continues Its Blog Series to Highlight Most Impactful Portfolio Companies With Champions Oncology, Inc. April 17th, 2014

Announcements

More effective kidney stone treatment, from the macroscopic to the nanoscale April 17th, 2014

High-temperature plasmonics eyed for solar, computer innovation April 17th, 2014

INSCX™ exchange to present Exchange trade reporting mechanism for engineered nanomaterials (NMs) to UK regulation agencies, insurers and upstream/downstream users April 17th, 2014

Transparent Conductive Films and Sensors Are Hot Segments in Printed Electronics: Start-ups in these fields show above-average momentum, while companies working on emissive displays such as OLED are fading, Lux Research says April 17th, 2014

Quantum Dots/Rods

Shiny quantum dots brighten future of solar cells: Photovoltaic solar-panel windows could be next for your house April 14th, 2014

Nanotech Business Review 2013-2014 April 9th, 2014

'Quantum Dots Market by Product (QD Displays, Lasers, Medical Devices, Solar Cells, Chip, Sensor), Application (Healthcare, Optoelectronics, Sustainable Energy), Material (Cadmium Selenide, Sulfide, Telluride), and Geography - Forecast & Analysis (2013 - 2020)' March 31st, 2014

Quantum Dots Take Center Stage at Inaugural Event: QD Vision Co-Founder and CTO Dr. Seth Coe-Sullivan to Chair First Quantum Dots Forum, March 26, 2014, San Diego, CA March 25th, 2014

Nanobiotechnology

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Biologists Develop Nanosensors to Visualize Movements and Distribution of Plant Stress Hormone April 15th, 2014

In latest generation of tiny biosensors, size isn't everything: UCLA researchers overturn conventional wisdom on nanowire-based diagnostic devices April 11th, 2014

Virus structure inspires novel understanding of onion-like carbon nanoparticles April 10th, 2014

NanoNews-Digest
The latest news from around the world, FREE







  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE