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Home > News > Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria

May 11th, 2009

Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria

Abstract:
To follow the entire process of antimicrobial action, we performed a variety of experiments including transmission electron microscopy and fluorescence correlation spectroscopy as well as single molecule tracking of quantum dot-labeled antimicrobial peptides on live bacteria. Since in vitro measurements do not necessarily correlate with the in vivo action of a peptide we developed a novel fluorescent live bacteria lysis assay.

Using fully functional nanoparticle-labeled Sushi 1, we observed the process of antimicrobial action at the single-molecule level.

Conclusion: Recently the hypothesis that many antimicrobial peptides act on internal targets to kill the bacterium has been discussed. Here, we demonstrate that the target sites of Sushi 1 are outer and inner membranes and are not cytosolic.

Source:
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