Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Cells traverse developmental divide via Blimp

Figure 1: Sox2 is among the genes upregulated by Blimp1 as part of the PGC developmental program. These cells have been fluorescently labeled to illustrate this process; Blimp1-expressing cells are labeled green, Sox2 protein is labeled red. In the right panel, cell nuclei have also been labeled with a generic staining agent and pseudo-colored white to reveal all individual cells in the specimen. Shown is a posterior part of a day 7.5 embryo.
Figure 1: Sox2 is among the genes upregulated by Blimp1 as part of the PGC developmental program. These cells have been fluorescently labeled to illustrate this process; Blimp1-expressing cells are labeled green, Sox2 protein is labeled red. In the right panel, cell nuclei have also been labeled with a generic staining agent and pseudo-colored white to reveal all individual cells in the specimen. Shown is a posterior part of a day 7.5 embryo.

Abstract:
A method for single-cell genomic profiling has helped researchers to identify a putative ‘master switch' for reproductive cell development in the mouse embryo

Cells traverse developmental divide via Blimp

Japan | Posted on October 31st, 2008

An animal's reproductive capabilities are established early in development, when a homogeneous embryonic cell population gives rise to two distinct cell types—somatic cells that form the vast majority of body tissues, and primordial germ cells (PGCs) that ultimately yield spermatozoa or ova.

Identifying genes responsible for ‘programming' PGC development will be essential to fully understand this essential developmental process. Unfortunately, existing techniques for large-scale gene expression profiling are designed for use with multicellular samples—an ineffective strategy for PGC analysis.

"PGCs are small in number—especially at early stages—and are embedded in somatic neighbors," explains Mitinori Saitou, of the RIKEN Center for Developmental Biology in Kobe. "Therefore, for systematically identifying genes specific to PGCs, single-cell analysis is considered to be essential." Prior work from Saitou's team identified several genes potentially important to PGC development. Now, his group has developed a powerful new technique for preparation and amplification of nucleic acids from individual cells, enabling stage-specific genomic profiling of mouse PGCs in unprecedented detail1.

The researchers focused on identifying genes regulated by Blimp1, a gene identified in their earlier work2. After analyzing PGCs from various developmental stages, it became clear that Blimp1 expression specifically increases in these cells over time. They also observed that although early-stage PGCs exhibit expression profiles for certain developmental genes that are similar to those observed in somatic cells, continued expression of Blimp1 leads to reversal of these expression patterns, actively driving development onto a PGC-specific trajectory (Fig. 1).

A broader comparison of stage-specific gene expression in PGCs and somatic cells enabled Saitou's team to assemble clusters of genes that are generally up- or down-regulated by Blimp1, allowing them to be categorized respectively as ‘specification' or ‘somatic' genes. Certain gene types were enriched for each category—including cell division regulators for the somatic genes and effectors of germ cell development for the specification genes—and each category also contained distinct sets of genes involved in embryonic development and body pattern formation.

Follow-up analyses confirmed that Blimp1 plays a central role in managing appropriate regulation of both somatic and specification genes for PGC development. "To me, the fact that Blimp1 represses essentially all the genes normally repressed in PGCs in comparison to their somatic neighbors is the most important finding," says Saitou. Now, having glimpsed the ‘big picture', Saitou's team hunting for the primary target genes for Blimp1, and the mechanism by which it switches them on to set PGC development in motion.
Reference

1. Kurimoto, K., Yabuta, Y., Ohinata, Y., Shigeta, M., Yamanaka, K. & Saitou, M. Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice. Genes & Development 22, 1617-1635 (2008).
2. Ohinata, Y., Payer, B., O'Carroll, D., Ancelin, K., Ono, Y., Sano, M., Barton, S.C., Obukhanych, T., Nussenzweig, M., Tarakhovsky, A., et al. Blimp1 is a critical determinant of the germ cell lineage in mice. Nature 436, 207-213 (2005).

The corresponding author for this highlight is based at the RIKEN Laboratory for Mammalian Germ Cell Biology

####

For more information, please click here

Copyright © Riken

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

article

Related News Press

News and information

Haydale Secures Exclusive Development and Supply Agreement with Tantec A/S: New reactors to be built and commissioned by Tantec A/S represent another step forward towards the commercialisation of graphene October 24th, 2014

QuantumWise guides the semiconductor industry towards the atomic scale October 24th, 2014

MEMS & Sensors Technology Showcase: Finalists Announced for MEMS Executive Congress US 2014 October 23rd, 2014

Nanoparticle technology triples the production of biogas October 23rd, 2014

Nanomedicine

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

Iranian Scientists Apply Nanotechnology to Produce Surgery Suture October 23rd, 2014

RF Heating of Magnetic Nanoparticles Improves the Thawing of Cryopreserved Biomaterials October 23rd, 2014

Sopping up proteins with thermosponges: Researchers develop novel nanoparticle platform that proves effective in delivering protein-based drugs October 22nd, 2014

Discoveries

QuantumWise guides the semiconductor industry towards the atomic scale October 24th, 2014

Iranian, Malaysian Scientists Study Nanophotocatalysts for Water Purification October 23rd, 2014

Nanoparticle technology triples the production of biogas October 23rd, 2014

Strengthening thin-film bonds with ultrafast data collection October 23rd, 2014

Announcements

Haydale Secures Exclusive Development and Supply Agreement with Tantec A/S: New reactors to be built and commissioned by Tantec A/S represent another step forward towards the commercialisation of graphene October 24th, 2014

QuantumWise guides the semiconductor industry towards the atomic scale October 24th, 2014

Advancing thin film research with nanostructured AZO: Innovnano’s unique and cost-effective AZO sputtering targets for the production of transparent conducting oxides October 23rd, 2014

Strengthening thin-film bonds with ultrafast data collection October 23rd, 2014

NanoNews-Digest
The latest news from around the world, FREE





  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE