Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > How to halt immune cell activation

Figure 1: Interaction between TCR, CD3 and LAPTM5. Left, CD3ζ (blue) is localized on the plasma membrane whereas LAPTM5 (green) and the lysosome-associated protein LAMP1 (red) are in the lysosomes in T cells before stimulation. Right, after TCR stimulation (α-CD3), CD3ζ moves to the lysosomal compartment where it co-localizes with LAPTM5 and LAMP1 and is degraded.
Reproduced, with permission, from Ref. 1 © 2008 Elsevier Inc.
Figure 1: Interaction between TCR, CD3 and LAPTM5. Left, CD3ζ (blue) is localized on the plasma membrane whereas LAPTM5 (green) and the lysosome-associated protein LAMP1 (red) are in the lysosomes in T cells before stimulation. Right, after TCR stimulation (α-CD3), CD3ζ moves to the lysosomal compartment where it co-localizes with LAPTM5 and LAMP1 and is degraded. Reproduced, with permission, from Ref. 1 © 2008 Elsevier Inc.

Abstract:
A new study sheds light on the molecular machinery required for reining in cellular signals that, if unleashed, could result in pathological inflammation

How to halt immune cell activation

Japan | Posted on October 10th, 2008

Researchers in Japan have identified part of the mechanism responsible for preventing prolonged—and potentially dangerous—activation of immune cells called T lymphocytes1. Each decorated with a unique surface receptor (TCR) capable of detecting pathogenic foreign proteins, T lymphocytes circulate throughout the body patrolling for invading microorganisms. Upon encounter with rogue proteins, TCRs trigger—via a complex of CD3 signaling proteins—intracellular events that orchestrate release of pro-inflammatory mediators called cytokines.

As unrestrained inflammation can cause tissue damage, the immune system exerts tight control over T lymphocyte activation. During healthy conditions, TCR and CD3 proteins are constantly internalized and released back to the lymphocyte surface; this ‘recycling' maintains a low level of TCR expression and thus a high ‘threshold' precluding unwarranted activation. After stimulation, however, TCRs and CD3 subunits are routed towards destructive intracellular compartments called lysosomes, where they are degraded as part of a signal ‘shut off' mechanism.

A team led by Ji-Yang Wang of the RIKEN Center for Allergy and Immunology in Yokohama sought to identify proteins underpinning this ‘fail safe' TCR signal termination process.

Having noted in previous experiments that expression of the lysosomal protein LAPTM5 is altered after TCR stimulation, the researchers tested whether LAPTM5 is involved in turning off TCR signals. They used genetic manipulation techniques to generate mutant mice in which the Laptm5 gene is not expressed. These Laptm5-deficient animals exhibited excessive T lymphocyte-driven responses to skin sensitization.

The team also found that, compared to normal T lymphocytes, LAPTM5-deficient T lymphocytes underwent more cell divisions, and released the cytokines interferon-γ and interleukin-2 more frequently after TCR stimulation. After activation, T lymphocytes lacking LAPTM5 expressed higher amounts of surface and intracellular TCR and a CD3 subunit, CD3ζ, than did wild-type T lymphocytes. Conversely, overexpression of LAPTM5 dampened CD3ζ expression.

TCR and CD3ζ proteins co-localized with LAPTM5 in lysosomes of activated T cells, and LAPTM5 physically interacted with CD3ζ (Fig. 1). These findings indicate that LAPTM5 might promote CD3ζ degradation by binding to and shuttling this protein to lysosomes.

Whether LAPTM5 cooperates with other lysosomal proteins to orchestrate CD3ζ destruction, and whether any human immune disorders are associated with mutations in Laptm5, remains to be determined.

LAPTM5 is the first lysosomal protein known to be specifically expressed in blood-generating (hematopoietic) cells. "In addition to its role in the negative regulation of TCR signaling, preliminary studies indicate that LAPTM5 may regulate the cell surface expression of additional immune receptors and may also function to prevent hematopoietic malignancies," says Wang.
Reference

1. Ouchida, R., Yamasaki, S., Hikida, M., Masuda, K., Kawamura, K., Wada, A., Mochizuki, S., Tagawa, M., Sakamoto, A., Hatano, M., Tokuhisa, T., Koseki, H., Saito, T., Kurosaki, T. & Wang, J.Y. A lysosomal protein negatively regulates surface T cell antigen receptor expression by promoting CD3ζ-chain degradation. Immunity 29, 33-43 (2008).

####

For more information, please click here

Copyright © Riken

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

article

Related News Press

News and information

Making graphene work for real-world devices: Fundamental research in phonon scattering helps researchers design graphene materials for applications April 24th, 2014

Return on investment for kit and promotion materials April 24th, 2014

Protecting olive oil from counterfeiters April 24th, 2014

NanoSafe, Inc. announces the addition of the Labconco Protector® Glove Box to its NanoSafe Tested™ registry April 23rd, 2014

Nanomedicine

Return on investment for kit and promotion materials April 24th, 2014

University of Tehran Researchers Invent Non-Enzyme Sensor to Detect Blood Sugar April 23rd, 2014

Gold nanoparticles help target, quantify breast cancer gene segments in a living cell April 23rd, 2014

QuantuMDx announce prototype handheld lab for 15 minute malaria diagnosis and drug resistance testing April 23rd, 2014

Discoveries

Making graphene work for real-world devices: Fundamental research in phonon scattering helps researchers design graphene materials for applications April 24th, 2014

Return on investment for kit and promotion materials April 24th, 2014

Protecting olive oil from counterfeiters April 24th, 2014

High-Performance, Low-Cost Ultracapacitors Built with Graphene and Carbon Nanotubes: Future devices based on technology could bridge gap between batteries and conventional capacitors in portable electronics and hybrid electric vehicles April 23rd, 2014

Announcements

Making graphene work for real-world devices: Fundamental research in phonon scattering helps researchers design graphene materials for applications April 24th, 2014

Return on investment for kit and promotion materials April 24th, 2014

Protecting olive oil from counterfeiters April 24th, 2014

High-Performance, Low-Cost Ultracapacitors Built with Graphene and Carbon Nanotubes: Future devices based on technology could bridge gap between batteries and conventional capacitors in portable electronics and hybrid electric vehicles April 23rd, 2014

NanoNews-Digest
The latest news from around the world, FREE







  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE