Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Nano-Softball Made of DNA

Can 20 trisoligonucleotides with 20×3 individual sequences be programmed to self-assemble into a DNA dodecahedron? The answer is yes if one starts from a new generation of trisoligonucleotides based on C3h-symmetric linkers with proper flexibility. The resulting dodecahedron has C1 symmetry and may facilitate the construction of multimodular scaffolds in the future.

Credit: (C) Wiley-VCH
Can 20 trisoligonucleotides with 20×3 individual sequences be programmed to self-assemble into a DNA dodecahedron? The answer is yes if one starts from a new generation of trisoligonucleotides based on C3h-symmetric linkers with proper flexibility. The resulting dodecahedron has C1 symmetry and may facilitate the construction of multimodular scaffolds in the future.
Credit: (C) Wiley-VCH

Abstract:
'Programmed' oligonucleotides with 3 branches organize themselves into dodecahedra

Nano-Softball Made of DNA

Germany | Posted on April 2nd, 2008

For quite some time, DNA, the stuff our genes are made of, has also been considered the building material of choice for nanoscale objects. A team led by Günter von Kiedrowski at the Ruhr University in Bochum has now made a dodecahedron (a geometric shape with twelve surfaces) from DNA building blocks. As reported in the journal Angewandte Chemie, these objects are formed in a self-assembly process from 20 individual trisoligonucleotides, building blocks consisting of a "branching junction" and three short DNA strands.

A regular dodecahedron is a geometric shape made of 12 pentagons of equal size, three of which are connected at every vertex. This results in a structure with 30 edges and 20 vertices. In order to produce a hollow dodecahedral object from DNA, the researchers used 20 "three-legged" building blocks (three DNA strands connected together at one point). The centers of these building blocks represent the vertices of the dodecahedron. The three edges projecting from each vertex are formed when a single strand of DNA converts two neighboring bridging components into a double strand.

In order for this process to result in a dodecahedron and not some other random geometric object, all of the DNA strands must have a different sequence. Among these, there must, however, be pairs of complementary strands that can bind to each other.

By using a computer program, the researchers identified a set of 30 independent, 15-base-pair-long, double-stranded DNA sequences with similar physical properties. The double-stranded sequences were assigned to the individual edges of the dodecahedron and to specific vertices for termination. It was then determined which three single-stranded sequences needed to be attached to each three-legged junction for the predetermined structure to form.

The team synthesized the 20 computed trisoligonucleotides by means of a solid-phase synthesis. The three DNA strands were always attached by way of an aromatic six-membered carbon ring. When mixed in equal parts in a buffer solution, these building blocks do aggregate to form the expected product: regular dodecahedra. Atomic force microscopy images reveal them to be uniform particles with a diameter of about 20 nm. Under pressure, the dodecahedra are quite flexible, the can be deformed like "soft balls" without incurring any damage.

If the trisoligonucleotides are equipped with pendant "arms", the dodecahedra can be outfitted with additional functional molecules. In this way, highly complex nanoconstructs, resembling little viruses in shape and size, should be accessible in the future. Potential applications range from medical diagnostics to nanoelectronics.

Author: Günter von Kiedrowski, Ruhr-Universität Bochum (Germany), http://www.ruhr-uni-bochum.de/oc1/mitarbeiter/Guenter-Kiedrowski.html
Title: Self-Assembly of a DNA Dodecahedron from 20 Trisoligonucleotides with C3h Linkers
Angewandte Chemie International Edition, doi: 10.1002/anie.200702682

####

For more information, please click here

Contacts:
Guenter von Kiedrowski

49-234-323-218

Copyright © Wiley-Blackwell

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

UTSA study describes new minimally invasive device to treat cancer and other illnesses: Medicine diffusion capsule could locally treat multiple ailments and diseases over several weeks December 3rd, 2016

Novel Electrode Structure Provides New Promise for Lithium-Sulfur Batteries December 3rd, 2016

Research Study: MetaSOLTM Shatters Solar Panel Efficiency Forecasts with Innovative New Coating: Coating Provides 1.2 Percent Absolute Enhancement to Triple Junction Solar Cells December 2nd, 2016

Deep insights from surface reactions: Researchers use Stampede supercomputer to study new chemical sensing methods, desalination and bacterial energy production December 2nd, 2016

Nanomedicine

UTSA study describes new minimally invasive device to treat cancer and other illnesses: Medicine diffusion capsule could locally treat multiple ailments and diseases over several weeks December 3rd, 2016

Nanobiotix Provides Update on Global Development of Lead Product NBTXR3: Seven clinical trials across the world: More than 2/3 of STS patients recruited in the “act.in.sarc” Phase II/III trial: Phase I/II prostate cancer trial now recruiting in the U.S. November 28th, 2016

From champagne bubbles, dance parties and disease to new nanomaterials: Understanding nucleation of protein filaments might help with Alzheimer's Disease and type 2 Diabetes November 24th, 2016

Nanopolymer-modified protein array can pinpoint hard-to-find cancer biomarker November 17th, 2016

Discoveries

UTSA study describes new minimally invasive device to treat cancer and other illnesses: Medicine diffusion capsule could locally treat multiple ailments and diseases over several weeks December 3rd, 2016

Novel Electrode Structure Provides New Promise for Lithium-Sulfur Batteries December 3rd, 2016

Research Study: MetaSOLTM Shatters Solar Panel Efficiency Forecasts with Innovative New Coating: Coating Provides 1.2 Percent Absolute Enhancement to Triple Junction Solar Cells December 2nd, 2016

Deep insights from surface reactions: Researchers use Stampede supercomputer to study new chemical sensing methods, desalination and bacterial energy production December 2nd, 2016

Announcements

UTSA study describes new minimally invasive device to treat cancer and other illnesses: Medicine diffusion capsule could locally treat multiple ailments and diseases over several weeks December 3rd, 2016

Novel Electrode Structure Provides New Promise for Lithium-Sulfur Batteries December 3rd, 2016

Research Study: MetaSOLTM Shatters Solar Panel Efficiency Forecasts with Innovative New Coating: Coating Provides 1.2 Percent Absolute Enhancement to Triple Junction Solar Cells December 2nd, 2016

Deep insights from surface reactions: Researchers use Stampede supercomputer to study new chemical sensing methods, desalination and bacterial energy production December 2nd, 2016

Nanobiotechnology

Deep insights from surface reactions: Researchers use Stampede supercomputer to study new chemical sensing methods, desalination and bacterial energy production December 2nd, 2016

Nanobiotix Provides Update on Global Development of Lead Product NBTXR3: Seven clinical trials across the world: More than 2/3 of STS patients recruited in the “act.in.sarc” Phase II/III trial: Phase I/II prostate cancer trial now recruiting in the U.S. November 28th, 2016

From champagne bubbles, dance parties and disease to new nanomaterials: Understanding nucleation of protein filaments might help with Alzheimer's Disease and type 2 Diabetes November 24th, 2016

Making spintronic neurons sing in unison November 18th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project