Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Fluorescent organic nanoparticles help illuminate cellular proteins

Abstract:
Like a smart highlighter, immunofluorescent labeling can zero in on a specific protein, helping scientists understand the structure of a cell and how diseases affect that structure. Current techniques have disadvantages, though.

Fluorescent organic nanoparticles help illuminate cellular proteins

ANN ARBOR, MI | Posted on March 8th, 2008

University of Michigan scientists developed a non-toxic, organic nanoparticle for immunofluorescent labeling that makes a bright, longer-lasting glow without the drawbacks of today's popular methods. A paper on the research will be published in the March 18 edition of the journal Advanced Materials.

"We've demonstrated the promising application of organic nanoparticles for immunofluorescent labeling," said Jinsang Kim, assistant professor of materials science and engineering who is the principal investigator of this research.

"Our molecules show unique properties. When they clump together, they get brighter, which is the opposite of what normally happens. Normally, when fluorescent molecules clump together, they become much dimmer, which is called self-quenching. Self-quenching is not a problem for our molecules."

Immunofluorescent labeling works like this: Scientists join fluorescent particles with protein-seeking molecules and let the companions loose in cells to bind to the protein they want to locate and study. The scientists then radiate the mixture with ultraviolet light. The light causes the fluorescent particles to glow, giving away the location of the protein the scientists were looking for.

Certain diseases can change the amount of particular proteins in cells. Prostate tumors, for example, can increase the level of prostate-specific antigen, or PSA, which is a cellular protein.

For fluorescent particles, scientists can currently choose between organic fluorescent dyes and inorganic quantum dots, both of which have shortcomings. Organic fluorescent dyes wear out easily from the ultraviolet light and inorganic quantum dots are toxic.

Kim's nanoparticles bridge the gap between these methods. They're non-toxic, and the researchers' novel way of making the nanoparticles causes them to shine brightly without deteriorating as easily as organic dyes.

Kim and his colleagues started by directing the self-assembly of a new kind of green fluorescent organic molecule called DBO. They mixed the fluorescent organic molecules in water together with a molecule called diacetylene that formed multi-layered bubbles around the fluorescent molecules and formed polymers. The fluorescent molecules glowed more than 12 times brighter in the multi-layered bubbles than they did in plain solution because of a unique arrangement of the molecules inside the bubbles.

The researchers tested their new nanoparticles by attaching them to biotin, a molecule that binds readily with the protein avidin. The researchers released the nanoparticles with biotin on a glass slide containing spots of avidin. The biotin found the avidin and Kim's nanoparticles glowed.

"More interestingly," Kim said, "the pressure-sensitive polydiacetylene bilayer surrounding the fluorescent nanoparticles also produced its own red fluorescence induced by the pressure the nanoparticles experienced when they attached to the target area. Green can't be seen through skin, but red can. This suggests additional applications for these nanoparticles."

The paper is called "Highly Emissive Self-assembled Organic Nanoparticles having Dual Color Capacity for Targeted Immunofluorescent Labeling."

Jinsang is also an assistant professor of Chemical Engineering, Macromolecular Science and Engineering and Biomedical Engineering. Other authors of the paper include: Hyong-Jun Kim, research fellow in Materials Science and Engineering; Jiseok Lee, graduate student in Macromolecular Science and Engineering.

####

About University of Michigan
The University of Michigan College of Engineering is ranked among the top engineering schools in the country. Michigan Engineering boasts one of the largest engineering research budgets of any public university, at more than $130 million annually. Michigan Engineering is home to 11 academic departments and a National Science Foundation Engineering Research Center. The college plays a leading role in the Michigan Memorial Phoenix Energy Institute and the Graham Environmental Sustainability Institute. Within the college, there is a special emphasis on research in three emerging areas: nanotechnology and integrated microsystems; cellular and molecular biotechnology; and information technology. Michigan Engineering is raising $300 million for capital projects and program support in these and other areas to continue fostering breakthrough scholarly advances, an unparalleled scope of student opportunities and contributions that improve the quality of life on an international scale.

For more information, please click here

Contacts:
Nicole Casal Moore
Phone: (734) 647-1838

Copyright © University of Michigan

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Haydale Secures Exclusive Development and Supply Agreement with Tantec A/S: New reactors to be built and commissioned by Tantec A/S represent another step forward towards the commercialisation of graphene October 24th, 2014

QuantumWise guides the semiconductor industry towards the atomic scale October 24th, 2014

MEMS & Sensors Technology Showcase: Finalists Announced for MEMS Executive Congress US 2014 October 23rd, 2014

Nanoparticle technology triples the production of biogas October 23rd, 2014

Nanomedicine

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

Iranian Scientists Apply Nanotechnology to Produce Surgery Suture October 23rd, 2014

RF Heating of Magnetic Nanoparticles Improves the Thawing of Cryopreserved Biomaterials October 23rd, 2014

Sopping up proteins with thermosponges: Researchers develop novel nanoparticle platform that proves effective in delivering protein-based drugs October 22nd, 2014

Discoveries

QuantumWise guides the semiconductor industry towards the atomic scale October 24th, 2014

Iranian, Malaysian Scientists Study Nanophotocatalysts for Water Purification October 23rd, 2014

Nanoparticle technology triples the production of biogas October 23rd, 2014

Strengthening thin-film bonds with ultrafast data collection October 23rd, 2014

Announcements

Haydale Secures Exclusive Development and Supply Agreement with Tantec A/S: New reactors to be built and commissioned by Tantec A/S represent another step forward towards the commercialisation of graphene October 24th, 2014

QuantumWise guides the semiconductor industry towards the atomic scale October 24th, 2014

Advancing thin film research with nanostructured AZO: Innovnano’s unique and cost-effective AZO sputtering targets for the production of transparent conducting oxides October 23rd, 2014

Strengthening thin-film bonds with ultrafast data collection October 23rd, 2014

NanoNews-Digest
The latest news from around the world, FREE





  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE