Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Nanoscale Dots Target and Track Apoptosis

Abstract:
Apoptosis, or programmed cell death, is a hallmark effect triggered by effective anticancer drugs. Now, researchers in Korea have developed a biocompatible, fluorescent nanoparticle that could provide an early sign that apoptosis is occurring as a result of anticancer therapy. The results of their work, published in the journal Bioconjugate Chemistry, could provide a boost for both clinical oncology and cancer research.

Nanoscale Dots Target and Track Apoptosis

Bethesda , MD | Posted on July 23rd, 2007

The availability of a real-time assay of apoptosis would provide a critically useful tool for oncologists, who would then have the means to determine whether a given therapeutic approach was working soon after that therapy was started. Cancer drug development would also benefit from a real-time apoptosis assay if such an assay could be used to provide rapid and sensitive results in high-throughput drug screening experiments.

A research team headed by Dae Hong Jeong, Ph.D., Yoon-Sik Lee, Ph.D., and Myung-Haing Cho, D.V.M., Ph.D., Seoul National University, created their fluorescent surface enhanced Raman spectroscopic (F-SERS) nanodots to boost the optical signal generated by typical, biocompatible fluorescent dyes. The nanodots consist of silver nanoparticles embedded in a silica sphere. Attached to the silica core are fluorescent dye molecules and molecules known as Raman labels that enhance the electronic interactions between the silver nanoparticles and the dye molecules. The researchers also linked annexin-V, a molecule that binds specifically to a chemical that appears on cells undergoing apoptosis, to the silica-silver nanoparticle construct.

Toxicity tests showed that the silica-silver nanodots were not toxic to various human cells growing in culture. The investigators then added the nanodots to cells triggered to undergo apoptosis and were able to image those cells as they went through programmed cell death. Based on these results, the researchers prepared other nanodots containing antibodies that bind to other molecules involved in apoptosis. They then added these antibody-linked nanodots and the annexin-V-linked nanodots to cultured human lung cancer cells. The investigators were able to track the appearance of all three molecules simultaneously, which has been difficult to do using conventional cell staining techniques.

This work is detailed in the paper "Multiplex targeting, tracking, and imaging of apoptosis by fluorescent surface enhanced raman spectroscopic dots." An investigator from the University of Illinois at Urbana-Champaign also participated in this study. An abstract of this paper is available through PubMed.

####

About National Cancer Institute
To help meet the goal of eliminating suffering and death due to cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Contacts:
National Cancer Institute
Office of Technology & Industrial Relations
ATTN: NCI Alliance for Nanotechnology in Cancer
Building 31, Room 10A49
31 Center Drive , MSC 2580
Bethesda , MD 20892-2580

Copyright © National Cancer Institute

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

View abstract

Related News Press

Nanomedicine

Researchers create vaccine for dust-mite allergies Main Page Content: Vaccine reduced lung inflammation to allergens in lab and animal tests July 22nd, 2014

NIST shows ultrasonically propelled nanorods spin dizzyingly fast July 22nd, 2014

SentiMag® Now Available in Australia and New Zealand July 21st, 2014

More than glitter: Scientists explain how gold nanoparticles easily penetrate cells, making them useful for delivering drugs July 21st, 2014

Discoveries

Iranian Scientists Produce Transparent Nanocomposite Coatings with Longer Lifetime July 24th, 2014

UCF Nanotech Spinout Developing Revolutionary Battery Technology: Power the Next Generation of Electronics with Carbon July 23rd, 2014

A Crystal Wedding in the Nanocosmos July 23rd, 2014

Nano-sized Chip "Sniffs Out" Explosives Far Better than Trained Dogs: TAU researcher's groundbreaking sensor detects miniscule concentrations of hazardous materials in the air July 23rd, 2014

Announcements

Silicene Labs Announces the Launch of Patent-Pending, 2D Materials Composite Index™ : The Initial 2D Materials Composite Index™ for Q2 2014 Is: 857.3; Founders Include World-Renowned Physicist and Seasoned Business and IP Professionals July 24th, 2014

Iranian Scientists Produce Transparent Nanocomposite Coatings with Longer Lifetime July 24th, 2014

Deadline Announced for Registration in 7th Int'l Nanotechnology Festival in Iran July 23rd, 2014

A Crystal Wedding in the Nanocosmos July 23rd, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE