Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Pairing Nanoparticles with Proteins

A cryo-electron micrograph showing a single layer of evenly spaced enzyme structures (colorful "wheels") interspersed with gold nanoparticles (magenta). Constructing such regular arrays of biomolecules might help develop high efficiency electrodes for biofuels or improve the resolution of cryo-electron microscopy in structural biology.
A cryo-electron micrograph showing a single layer of evenly spaced enzyme structures (colorful "wheels") interspersed with gold nanoparticles (magenta). Constructing such regular arrays of biomolecules might help develop high efficiency electrodes for biofuels or improve the resolution of cryo-electron microscopy in structural biology.

Abstract:
New ways to tag, engineer molecules for energy conversion, drug delivery, and medical imaging

Pairing Nanoparticles with Proteins

UPTON, NY | Posted on June 27th, 2007

In groundbreaking research, scientists have demonstrated the ability to strategically attach gold nanoparticles -- particles on the order of billionths of a meter -- to proteins so as to form sheets of protein-gold arrays. The nanoparticles and methods to create nanoparticle-protein complexes can be used to help decipher protein structures, to identify functional parts of proteins, and to "glue" together new protein complexes. Applications envisioned by the researchers include catalysts for converting biomass to energy and precision "vehicles" for targeted drug delivery.

The research, which was conducted at the U.S. Department of Energy's Brookhaven National Laboratory, will be published in the July 2, 2007 issue of the journal Angewandte Chemie.

"Our study demonstrates that nanoparticles are appealing templates for assembling functional biomolecules with extensive potential impact across the fields of energy conversion, structural biology, drug delivery, and medical imaging," said lead author Minghui Hu, a postdoctoral student working with James Hainfeld, Raymond Brinas, Luping Qian, and Elena Lymar in the Biology Department at Brookhaven Lab.

In the field of energy conversion, scientists have been searching for efficient ways to convert organic fuels such as ethanol into electricity using catalytic electrodes. But making single layers of densely packed enzymes, the functional part of such catalytic electrodes, has been a challenge. This new research shows that precisely engineered gold nanoparticles can be used to "glue" enzymes together to form oriented and ordered single layers, and that these monolayers are mechanically stable enough to be transferred onto a solid surface such as an electrode.

For this research, the scientists attached gold nanoparticles to an enzyme complex that helps drug-resistant tuberculosis bacteria survive, which has been studied by Brookhaven Lab biologist Huilin Li. The researchers suggest that gold nanoparticles might also be tailored to inactivate this enzyme complex, thereby thwarting drug-resistant TB -- a research avenue they may explore in future studies.

In another part of the study, the researchers used proteins found on the surface of adenovirus, a virus that causes the common cold. Previous studies by Broookhaven's Paul Freimuth have characterized how this virus binds to the human cells it infects, and have suggested that modified forms of adenovirus could be used as vehicles to deliver drugs to specific target cells, such as those that make up tumors.

One key to this approach would be to enhance strong binding to the target cells. Toward that end, Hu and Hainfeld's group attached multiple viral proteins to the gold nanoparticles. Such constructs should have increased binding affinity for target cells and their larger size should extend blood residence time for improved drug delivery.

In another application, this new research showed that gold nanoparticles can enhance scientists' ability to decipher the structures and functionally important regions of protein molecules - the workhorses that carry out every function of living cells and whose dysfunction often leads to disease. With added nanoparticles, the "signal-to-noise ratio" and resolution of an imaging technique known as cryo-electron microscopy were significantly increased. This method might enable analysis of small biological macromolecules and complexes that are currently intractable to analyze by cryo-electron microscopy or x-ray crystallography.

Throughout this work, the biggest challenge was to synthesize size-controllable nanoparticles coated with organic molecules designed to react with specific protein sites. Hu explains the steps: "First, we design the specific interactions between gold nanoparticles and the proteins by coating the gold nanoparticles with functional organic molecules using a biocompatible linker. Then we add a genetically engineered sequence of peptides, called a "tag," to the protein molecule, which acts as the binding site for the gold nanoparticles. Finally, we incubate the nanoparticles with the protein solution to allow the nanoparticles and proteins to bind, transfer the solution onto a transmission electron microscopy grid, and analyze the complexes using state-of-the-art electron microscopes."

####

About Brookhaven National Laboratory
This research was funded by Brookhaven's Laboratory Directed Research and Development program, the Office of Environmental and Biological Research within the U.S. Department of Energy's Office of Science, and by the Institute of General Medical Sciences within the National Institutes of Health. Collaborators on the research include: Huilin Li, Guiqing Hu, Yanbiao Zhang, and Paul Freimuth, all from the Biology Department at Brookhaven, and Joseph Wall, Martha Simon, Beth Lin, and Frank Kito of the Brookhaven Lab scanning transmission electron microscope (STEM) team.

One of ten national laboratories overseen and primarily funded by the Office of Science of the U.S. Department of Energy (DOE), Brookhaven National Laboratory conducts research in the physical, biomedical, and environmental sciences, as well as in energy technologies and national security. Brookhaven Lab also builds and operates major scientific facilities available to university, industry and government researchers. Brookhaven is operated and managed for DOE's Office of Science by Brookhaven Science Associates, a limited-liability company founded by the Research Foundation of State University of New York on behalf of Stony Brook University, the largest academic user of Laboratory facilities, and Battelle, a nonprofit, applied science and technology organization.

Visit Brookhaven Lab's electronic newsroom for links, news archives, graphics, and more: http://www.bnl.gov/newsroom
--
Media & Communications Office (631) 344-2345 phone
Community, Education, Government (631) 344-8350 phone
& Public Affairs Directorate (631) 344-3368 fax
Brookhaven National Laboratory
Upton NY 11973 http://www.bnl.gov

For more information, please click here

Contacts:
Karen McNulty Walsh

(631)344-8350
or
Mona Rowe

(631) 344-5056

Copyright © Brookhaven National Laboratory

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

Nanomedicine

Spinning strands hint at folding dynamics: Rice University lab uses magnetic beads to model microscopic proteins, polymers October 17th, 2017

Arrowhead Pharmaceuticals to Present Preclinical Data on ARO-AAT at The Liver Meeting(R) October 10th, 2017

Arrowhead to Present at Chardan Gene Therapy Conference October 3rd, 2017

'CRISPR-Gold' fixes Duchenne muscular dystrophy mutation in mice October 3rd, 2017

Discoveries

A step closer to understanding quantum mechanics: Swansea Universityís physicists develop a new quantum simulation protocol October 22nd, 2017

Creation of coherent states in molecules by incoherent electrons October 21st, 2017

Novel 'converter' heralds breakthrough in ultra-fast data processing at nanoscale: Invention bagged four patents and could potentially make microprocessor chips work 1,000 times faster October 20th, 2017

Strange but true: turning a material upside down can sometimes make it softer October 20th, 2017

Announcements

A step closer to understanding quantum mechanics: Swansea Universityís physicists develop a new quantum simulation protocol October 22nd, 2017

Creation of coherent states in molecules by incoherent electrons October 21st, 2017

Strange but true: turning a material upside down can sometimes make it softer October 20th, 2017

Leti Coordinating Project to Develop Innovative Drivetrains for 3rd-generation Electric Vehicles: CEA Techís Contribution Includes Litenís Knowhow in Magnetic Materials and Simulation And Letiís Expertise in Wide-bandgap Semiconductors October 20th, 2017

Energy

New nanomaterial can extract hydrogen fuel from seawater: Hybrid material converts more sunlight and can weather seawater's harsh conditions October 4th, 2017

Researchers set time limit for ultrafast perovskite solar cells September 22nd, 2017

Copper catalyst yields high efficiency CO2-to-fuels conversion: Berkeley Lab scientists discover critical role of nanoparticle transformation September 20th, 2017

Solar-to-fuel system recycles CO2 to make ethanol and ethylene: Berkeley Lab advance is first demonstration of efficient, light-powered production of fuel via artificial photosynthesis September 19th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project