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Home > Press > New 'chromatography flow-mode' option transforms Zetasizer Nano

Abstract:
Launched at Pittcon 2007 (25 Feb - 2 Mar 07; Chicago, USA) the new ‘chromatography flow-mode' option for Malvern's market-leading Zetasizer Nano particle and molecular characterization system, enables its use as an in-line chromatography detector for protein applications.

New 'chromatography flow-mode' option transforms Zetasizer Nano

MALVERN, UK | Posted on February 26th, 2007

A significant new development, ‘chromatography flow-mode' permits the coupling of dynamic light scattering with size exclusion chromatography, conferring the ability to perform Absolute Size Exclusion Chromatography (ASEC). This allows protein size to be measured directly rather than relying on column calibration or additional data from a second detector, and means that molecular weight standards are no longer required to identify oligomeric species or determine protein purity.

To use the Zetasizer Nano as an absolute light scattering detector it simply needs to be connected after the last detector in a size exclusion chromatography (SEC) system. The instrument can be added to any SEC set-up; no column calibration is required and the Zetasizer Nano's software makes the entire measurement process simple and straightforward. For the majority of protein separations in aqueous media, no extra parameters are required. Size is plotted in real time as material is eluted from the column, and the molecular weight of each peak is calculated automatically at the end of the measurement.

The Zetasizer Nano was designed specifically to meet the low concentration and small sample volume requirements of protein and other biomolecular applications and is now in widespread use. It is by applying the system's patented optical design and high sensitivity capabilities to real time flow measurements that Malvern has facilitated the Zetasizer Nano's use as a chromatography detector, providing a major new tool for protein researchers.

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About Malvern Instruments Inc
Malvern Instruments is a global company that develops, manufactures and markets advanced analytical systems used in characterizing a wide variety of materials, from bulk powders to the latest nanomaterials and delicate macromolecules. Innovative technologies and powerful software produce systems that deliver industrially relevant data enabling customers to make the connection between micro (eg particle size) and macro (bulk) material properties (rheology) and chemical composition (chemical imaging). Malvern laboratory, on-line and in-line solutions are proven in sectors from cement to pharmaceuticals and support the understanding, improvement and optimization of many industrial processes.

Headquartered in Malvern, UK, the company has subsidiary organizations in all major European markets, North America, China, Korea and Japan, key partnerships in India, a global distributor network and a number of applications laboratories around the world.

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Contacts:
Trish Appleton
Kapler Communications
Suite 2, Cressner House
12 Huntingdon Street
St Neots, Cambs PE19 1BD UK
Tel: +44 (0) 1480 471117
Fax: +44 (0) 1480 471118

USA contact:
Marisa Fraser
Malvern Instruments Inc
10 Southville Road
Southborough, MA 01772, USA
Tel: +1 508 480 0200
Fax: +1 508 460 9692


Please send sales enquiries to:
Alison Vines
Malvern Instruments Ltd
Enigma Business Park, Grovewood Road, Malvern, Worcestershire WR14 1XZ UK
Tel: +44 (0) 1684 892456
Fax: +44 (0) 1684 892789

Copyright © Malvern Instruments Inc

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