Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Mini-beacon design a major development for smart drug delivery

Abstract:
A nanoscopic beacon used by Melbourne researchers will help to enhance the design of smart gene and drug delivery systems.

Mini-beacon design a major development for smart drug delivery

August 02, 2005

A team from the University of Melbourne’s Department of Chemical and Biomolecular Engineering has used a molecular beacon made from single DNA strands to measure how easily DNA (e.g. genes) can pass through the wall of drug delivery particles.

Federation Fellow Professor Frank Caruso, who heads the Centre for Nanoscience and Nanotechnology, says, “The past number of years has seen major advances in the design of ‘molecular vehicles’ – particles that can be filled with a medicine or new genes. The vehicles then ferry their contents to the site in the body where they are needed.”

“One of the major roadblocks that we have encountered in designing these molecular transport systems is how to get the vehicle contents out of their container once they reach the site where they are needed.”

In order to achieve this effectively, the researchers need to know how big the pores in the vehicle’s membranes are and how easily the contents can pass through them. This has proved quite difficult.

Dr. Angus Johnston who works with Professor Caruso says, “Scientists designing these drug-delivery vehicles need to be able to measure the very small number of molecules which pass through the membrane. Normally, we could label the molecules, so we can see them as they pass through. The problem with this is that adding a label alters the size, so the ability to pass through the pore will change when the label is removed.”

Dr. Johnston and Professor Caruso have developed a clever technique that overcomes this problem which allows scientists to rapidly and accurately determine the permeability of DNA through films.

The beacons the researchers used are single DNA strands which have a light-emitting molecule (a fluorophore) at one end and a quencher at the other. A fluorophore is simply a molecule that emits light and a quencher is a molecule that stops the fluorophore from emitting light.

The DNA strand self assembles so that the two end segments pair up, forming a loop in the centre – much like the shape of a round-bottomed flask. This is the closed molecular beacon.

When the beacon is closed the fluorophore on one end of the DNA strand is close to the “quencher” on the other end, which stops the fluorophore from giving off light.

To determine the permeability of the capsule, the molecular beacons are placed inside the delivery vehicle. If DNA passes through the capsule wall, the beacon opens and the fluorophore emits light. So when DNA passes through the capsule, the beacon is switched ‘on.’ If no DNA passes through the capsule, the beacon remains switched off.

The researchers used this technique with different length strands of DNA and were able to use the beacons to successfully determine whether or not the strands were able to pass through the membrane.

Professor Caruso says, “We hope to now use the technique in the design of intelligent drug-delivery systems which can transport medicine to target locations and release the contents in a controlled way.”

The research was recently published in the Journal of the American Chemical Society and highlighted in the July 15 Issue of the journal Science. This research was funded by the Australian Research Council.

####

Contact:
Elaine Mulcahy
Media Promotions Officer
emulcahy@unimelb.edu.au
Tel: 61 3 8344 0181
Mob: 0421 641 506

Frank Caruso
Chemical and Biomolecular Engineering
(03) 8344 3461
fcaruso@unimelb.edu.au

Angus Johnston
Chemical and Biomolecular Engineering
(03) 8344 9833
0409 863 255
angusj@unimelb.edu.au

Copyright © University of Melbourne

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

Self Assembly

Live Images from the Nano-cosmos: Researchers watch layers of football molecules grow November 5th, 2014

Outsmarting Thermodynamics in Self-assembly of Nanostructures: Berkeley Lab reports method for symmetry-breaking in feedback-driven self-assembly of optical metamaterials November 4th, 2014

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

NIST offers electronics industry 2 ways to snoop on self-organizing molecules October 22nd, 2014

Nanomedicine

Research reveals how our bodies keep unwelcome visitors out of cell nuclei November 24th, 2014

ASU, IBM move ultrafast, low-cost DNA sequencing technology a step closer to reality November 24th, 2014

An Inside Job: UC-Designed Nanoparticles Infiltrate, Kill Cancer Cells From Within November 24th, 2014

Iran Exports Nanodrugs to Syria November 24th, 2014

Sensors

Cooling with the coldest matter in the world November 24th, 2014

Canatu Launches CNB In-Mold Film for Transparent Touch on 3D Surfaces –in Cars, Household Appliances, Wearables, Portables November 20th, 2014

UO-industry collaboration points to improved nanomaterials: University of Oregon microscope puts spotlight on the surface structure of quantum dots for designing new solar devices November 20th, 2014

Spiraling light, nanoparticles and insights into life’s structure November 19th, 2014

Discoveries

Researchers engineer improvements of technology used in digital memory November 24th, 2014

Research reveals how our bodies keep unwelcome visitors out of cell nuclei November 24th, 2014

ASU, IBM move ultrafast, low-cost DNA sequencing technology a step closer to reality November 24th, 2014

An Inside Job: UC-Designed Nanoparticles Infiltrate, Kill Cancer Cells From Within November 24th, 2014

Announcements

Research reveals how our bodies keep unwelcome visitors out of cell nuclei November 24th, 2014

ASU, IBM move ultrafast, low-cost DNA sequencing technology a step closer to reality November 24th, 2014

An Inside Job: UC-Designed Nanoparticles Infiltrate, Kill Cancer Cells From Within November 24th, 2014

Cooling with the coldest matter in the world November 24th, 2014

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More












ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE